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Analysis of Interactions of DNA polymerase β and reverse transcriptases of human immunodeficiency and mouse leukemia viruses with dNTP analogs containing a modified sugar residue

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Abstract

Substrate properties of various morpholinonucleoside triphosphates in the reaction of DNA elongation catalyzed by DNA polymerase β, reverse transcriptase of human immunodeficiency virus (HIV-1 RT), and reverse transcriptase of Moloney murine leukemia virus (M-MuLV RT) were compared. Morpholinonucleoside triphosphates were utilized by DNA polymerase β and HIV-1 reverse transcriptase as substrates, which terminated further synthesis of DNA, but were virtually not utilized by M-MuLV reverse transcriptase. The kinetic parameters of morpholinoderivatives of cytosine (MorC) and uridine (MorU) were determined in the reaction of primer elongation catalyzed by DNA polymerase β and HIV-1 reverse transcriptase. MorC was a more effective substrate of HIV-1 reverse transcriptase and significantly less effective substrate of DNA polymerase β than MorU. The possible use of morpholinonucleoside triphosphates as selective inhibitors of HIV-1 reverse transcriptase is discussed.

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Authors and Affiliations

  1. Siberian Division of the Russian Academy of Sciences, Institute of Chemical Biology and Fundamental Medicine, pr. Lavrentieva 8, 630090, Novosibirsk, Russia

    N. A. Lebedeva, V. N. Silnikov, T. V. Abramova, A. S. Levina, S. N. Khodyreva, N. I. Rechkunova & O. I. Lavrik

  2. Novosibirsk State University, ul. Pirogova 2, 630090, Novosibirsk, Russia

    T. A. Seredina

Authors
  1. N. A. Lebedeva
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  2. T. A. Seredina
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  3. V. N. Silnikov
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  4. T. V. Abramova
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  5. A. S. Levina
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  6. S. N. Khodyreva
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  7. N. I. Rechkunova
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  8. O. I. Lavrik
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Correspondence to O. I. Lavrik.

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Translated from Biokhimiya, Vol. 70, No. 1, 2005, pp. 5–13. Original Russian Text Copyright © 2005 by Lebedeva, Seredina, Silnikov, Abramova, Levina, Khodyreva, Rechkunova, Lavrik.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM04-241, December 5, 2004.

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Lebedeva, N.A., Seredina, T.A., Silnikov, V.N. et al. Analysis of Interactions of DNA polymerase β and reverse transcriptases of human immunodeficiency and mouse leukemia viruses with dNTP analogs containing a modified sugar residue. Biochemistry (Moscow) 70, 1–7 (2005). https://doi.org/10.1007/PL00021748

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  • DOI: https://doi.org/10.1007/PL00021748

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