Abstract
The ostrich fern,Matteuccia struthiopteris L., contains two globulin spore storage proteins of 2.2 S and 11.3 S, with physical characteristics similar to those of seed storage proteins ofBrassica napus (rapeseed) andRaphanus sativus (radish). By the use of a cloned cDNA that encodes the 1.7 SB. napus storage protein (napin), gene sequences that hybridized with napin were detected in fern nuclear DNA, and a 900-nucleotide homologous mRNA was detected in developing spores.In vitro translation of this fern mRNA produced a 22-kD polypeptide comparable in size to the 21-kD precursor polypeptide identified inBrassica. No hybridizations were observed between theBrassica 12 S clone and either fern DNA or developing spore mRNA.
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This work was supported in part by a Grant-in-Aid of Research to T.S.T. from Sigma XI, an award from the Research Committee of Dartmouth College to A.E.D., and a William and Flora Hewlett Foundation grant of the Research Corporation to D.B.S.
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Templeman, T.S., Stein, D.B. & DeMaggio, A.E. A fern spore storage protein is genetically similar to the 1.7 S seed storage protein ofBrassica napus . Biochem Genet 26, 595–603 (1988). https://doi.org/10.1007/PL00020499
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DOI: https://doi.org/10.1007/PL00020499