Growth Performance and Gonadal Development of Growth Enhanced Transgenic Tilapia Oreochromis niloticus (L.) Following Heat-Shock-Induced Triploidy

Abstract:

Triploid induction offers a way of considerably reducing fertility in fish, and could therefore be employed to help ensure that any adverse environmental impact of transgenic fish was markedly less. In order to produce sterile growth-enhanced transgenic fish, we have induced triploidy in two lines of transgenic tilapia. Growth performance and gonadal development were analyzed following triploidization by heat shock. Ploidy status was confirmed by nuclear size measurement of erythrocytes. Erythrocytes of triploids were found to be 1.5 times larger than diploids. Observations of growth enhancement and gonadal development were made on diploids and triploids from both transgenic and nontransgenic full sibling batches. In both lines, transgenic diploids were superior in growth performance, followed by transgenic triploids, nontransgenic diploids, and nontransgenic triploids. Although the testes of transgenic triploids were significantly smaller than those of nontransgenic triploids and nontransgenic diploids, histologically they did not show signs of gross deformation. There were also some spermatozoa present in the testes of some triploids, which could be indicative of reproductive functionality. However, the ovaries were devoid of oocytes, underdeveloped, and deformed in all triploids and were completely nonfunctional. Although the best growth performance was shown by the fertile diploid transgenics, the triploid transgenic females could offer a good option for aquaculture purposes because they showed superior growth performance over the normal wild-type tilapias with the advantage of sterility to ensure nonhybridization and noncontamination with the local gene pool. However, careful monitoring of potential gene flow would be required prior to commercial use.