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Factors Influencing T-DNA Transfer from Agrobacterium to Precultured Immature Wheat Embryos (Triticum Aestivum L.)

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Summary

We have studied several factors influencing the transfer of the T-DNA from Agrobacterium to wheat cells. Agrobacterium tumefaciens and A. rhizogenes strains were screened using immature zygotic embryos from the two wheat varieties Bobwhite and Lona (Triticum aestivum L.). Transient T-DNA transfer to plant cells was shown using a β-glucuronidase (gus)-intron marker gene. A. rhizogenes LBA9402 transferred the T-DNA efficiently to wheat cells, where it was highly expressed. The efficiency of T-DNA transfer was influenced by the cocultivation time, the Agrobacterium concentration and the preculture time of the isolated immature embryos before inoculation.

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Authors and Affiliations

  1. ETH Zurich, Institute of Plant Sciences, Universitätstr. 2, CH-8092, Zurich, Switzerland

    Murielle Uzé, Ingo Potrykus & Christof Sautter

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  1. Murielle Uzé
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  2. Ingo Potrykus
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  3. Christof Sautter
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Correspondence to Christof Sautter.

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Uzé, M., Potrykus, I. & Sautter, C. Factors Influencing T-DNA Transfer from Agrobacterium to Precultured Immature Wheat Embryos (Triticum Aestivum L.). CEREAL RESEARCH COMMUNICATIONS 28, 17–23 (2000). https://doi.org/10.1007/BF03543569

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  • DOI: https://doi.org/10.1007/BF03543569

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