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Assessment of Tissue Culture — Derived Durum Wheat Lines for Somaclonal Variation

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Abstract

Progeny R3 of 8 regenerated in in vitro culture durum wheat lines from cultivar Progress were analyzed and compared to the initial genotype for agronomical and physiological traits. The plants were regenerated in culture from mature embryo and immature inflorescences on MS basal medium. The resistance of plants to abiotic stress was estimated through an express method of growth depression of seedlings placed in stress conditions. Significant differences between regenerated plants and controls for both agronomical traits and cold- and drought resistance were found. The coefficient of variation for most traits was higher in population of plants regenerated from immature inflorescences than in that from mature embryos. By cluster analysis was estimated that lines R5, R4 and R3 regenerated in culture from immature inflorescences were the most phenotype and genotype distant from initial genotype.

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References

  • Adlons, S, Kunanuvatchidach, R. and Godwin, I., 1995, Somaclonal variation in rice - drought tolerance and other agronomic characters, Aust. J. of Bot., 43, 201–209

    Article  Google Scholar 

  • Bailie, A., Rossnagel, B. and Kartha, K., 1992, Field evaluation of barley genotypes derived from tissue culture, Can. J. Plant Sci., 72, 725–733

    Article  Google Scholar 

  • Bertin, P. and Bouharmont, J., 1997, Use of somaclonal variation and in vitro selection for chilling tolerance improvement in rice, Euphytica, 96, 135–142

    Article  Google Scholar 

  • Blum, A., Sinmena, B. and Ziv, O., 1980, An evaluation of seed and seedling drought tolerance screening test in wheat, Euphytica, 29, 727–736

    Article  Google Scholar 

  • Borja, J., Abdalla, O., Mujeeb-Kazi, A., Ter-Kuile, N. and Autrique, E., 1994, Agronomic evaluation of somaclonal variants from durum wheat cultivars, Agron. Abstr. American Society of Agronomy, Madison

    Google Scholar 

  • Bozhanova, V., 1997, Investigation of durum wheat drought resistance through seedling growth depressing at osmotic stress (Bulgarian), Proceeding from Second scientific conference “Fibre and cereal crop problem“, Chirpan, 78–83

    Google Scholar 

  • Bozhanova, V. and Petrova, T., 2000, Cold resistance estimation of durum wheat genotypes (Bulgarian), Plant Science, Sofia, 37, 705–707

    Google Scholar 

  • Bozorgipour, R. and Snape, J., 1997, An assessment of somaclonal variation as a breeding tool for generating herbicide tolerant genotypes in wheat, Euphytica, 94, 335–340

    Article  Google Scholar 

  • Bregitzer, P. and Poulson, M., 1995, Agronomic performance of barley lines derived from tissue culture, Crop. Sci., 35, 1144–1148

    Article  Google Scholar 

  • Encheva, U., 2001, Production and study of initial breeding material from sunflower by using in vitro techniques, Ph.D. Thesis, Wheat and Sunflower Institute, Dobrich, Bulgaria

    Google Scholar 

  • Evans, D. and Sharps, W., 1983, Single gene mutations in tomato plants regenerated from tissue culture, Science, 221, 949–951

    Article  CAS  Google Scholar 

  • Galiba, G., Kertesz, Z., Sutka, J. and Sagi, L., 1985, Differences in somaclonal variation in three winter wheat varieties, Cereal Res. Comm., 13, 345–350

    Google Scholar 

  • Galiba, G. and Sutka, J., 1989, Frost resistance of somaclones derived from Triticum aestivum L. winter calli, Plant Breeding, 102, 101–104

    Article  Google Scholar 

  • Gamborg, O., Miller, R., and Ojma, K., 1968, Nutrient requirements of suspension cultures of soybeen root cells, Exp. Cell Res., 50, 148–151

    Article  Google Scholar 

  • Hanson, K., Hucl, P. and Backer, R., 1994, Comparative field performance of tissue culture derived lines and breed lines of HY 320 spring wheat, Plant Breeding, 112, 183–191

    Article  Google Scholar 

  • Kasha, K., Ziauddin, A., Reinbergs, E. and Falk, D, 1991, Use of haploids in induced mutation in barley and wheat, Cereal Res. Commun, 1–2, 101–108

    Google Scholar 

  • Larkin, P. and Scowcroft, W., 1981, Somaclonal variation - a novel source of variability from cell cultures for plant improvement, Theor. Appl. Genet., 60, 197–214

    Article  CAS  Google Scholar 

  • Larkin, P., Ryan, S., Brettel, J. and Scowcroft, W., 1984, Heritable somaclonal variation in wheat, Theor. Appl. Genet., 67, 443–455

    Article  CAS  Google Scholar 

  • Lörz, H., Becker, D. and Luetticke, S., 1998, Molecular wheat breeding by direct gene transfer, Euphytica, 100, 219–223

    Article  Google Scholar 

  • Milkova, V., Ivanov, P, Atanasov, Z., Todorov, I., Panayotov, I., 1995, Callus culture and gamma ray treatment used for inducing new breeding material in wheat, Biotechnol. and Biotechnol. Eq., 9, 2, 31–36

    Article  Google Scholar 

  • Mohmand, A. and Nabors, M., 1990, Somaclonal variant plants of wheat derived from mature embryo expiants of three genotypes. Plant Cell Reports, 8, 558–560

    Article  CAS  Google Scholar 

  • Murashige, T. and Skoog, F., 1962, A revised medium for rapid growth and bioassays with tobacco tissue culture, Physiol. Plant., 15, 473–497

    Article  CAS  Google Scholar 

  • Ryan, S., Larkin, P. and Ellison, F., 1987, Somaclonal variation in some agronomic and quality characters in wheat, Theor. Appl. Genet., 74, 77–82

    Article  CAS  Google Scholar 

  • Symillides, Y, Henry, Y. and De Buyser, J, 1995, Analysis of Chinese Spring regenerants obtained from short- and long-term wheat somatic embryogenesis, Euphytica, 82, 263–268

    Article  Google Scholar 

  • Udovenko, G. and Goncharova, E., 1982, Effective express methods for screening genotype- and individual resistance of plant to extremal conditions, Reports of VASHNIL, 7, 13–15

    Google Scholar 

  • Villareal, R., Ter-Kuile, N., Del Toro, E., Rajaram, S. and Mujeeb-Kazi, A., 1993, Agronomic evaluation of somaclonal variants from three wheat cultivars, Agron. Abstr. American Society of Agronomy, Madison

    Google Scholar 

  • Villareal, R., Mujeeb-Kazi, A and Pena, R., 1999, Agronomic performance and quality characteristics of tissue culture-derived lines of spring wheat cultivar Pavon, Cereal Res. Comm., 27, 1–2, 41–48

    Google Scholar 

  • Ward, J., 1963, Hierarchical grouping to optimize an objective function, J. Am. Stat. Assoc, 58, 236–244

    Article  Google Scholar 

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Correspondence to Violeta Bozhanova.

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Bozhanova, V., Dechev, D. Assessment of Tissue Culture — Derived Durum Wheat Lines for Somaclonal Variation. CEREAL RESEARCH COMMUNICATIONS 30, 277–284 (2002). https://doi.org/10.1007/BF03543419

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