Abstract
Capillary electrophoresis (CE) of gliadins from Triticum species in acidic or isoelectric buffers is currently used. The performance of five buffers are compared here. The highest resolving power and the shortest time of analysis resulted associated to the isoelectric buffers. The slight different performance of the iminodiacetic acid (IDA) and aspartic acid (Asp) buffer systems should be taken into consideration before to start CE analyses. IDA buffer should be preferred when screening large germplasm collections or when a detailed characterisation of ω-gliadins is required. Asp buffer proved to be most satisfactory for cultivar discrimination or varietal identification.
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Abbreviations
- ACN:
-
acetonitrile
- Asp:
-
aspartic acid
- CE:
-
capillary electrophoresis
- CZE:
-
capillary zone electrophoresis
- HEC:
-
2-hydroxyethyl cellulose
- HPMC:
-
hydroxypropyl-methyl cellulose
- IDA:
-
iminodiacetic acid
- PAGE:
-
polyacrylamide gel electrophoresis
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Piergiovanni, A.R. General experimental aspects for capillary electrophoretic analysis of gliadins in Triticum species. CEREAL RESEARCH COMMUNICATIONS 32, 395–402 (2004). https://doi.org/10.1007/BF03543326
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DOI: https://doi.org/10.1007/BF03543326