Abstract
Rice genotypes were evaluated for salt tolerance in a greenhouse based upon stable carbon isotope analysis under salt stress (EC = 8 dSm−1) and non-stressed (1 dSm−1) conditions. Ten seedlings from each replicate and genotype were randomly sampled at 25 or 33 days after sowing (DAS). Values for carbon isotope discrimination (A) were obtained from dried and homogenized seedling tissue of twelve genotypes. Rice genotypes were ranked from high to low salt tolerance based on whole plant average maximum A of these two salt levels at these two early growth stages. Differences in fractionation among genotypes were observed at 25 and 33 DAS. Fractionation effects of salinity stress on A were significantly different among genotypes at 33 DAS, but not at 25 DAS. The salt tolerant cultivar maintained significantly higher average flag leaf tissue A than did the sensitive cultivar. Flag leaves of salt stressed rice maintained higher A than predicted for these two genotypes based a theoretical A value. The lack of greater reductions in leaf tissue A of salt stressed rice genotypes may the result of an increased contribution of respired carbon to the total carbon budget of rice leaves.
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Asch, F., M. Dingkuhn, and K. Dorffling. 2000. Salinity increases CO2 assimilation but reduces growth in field-grown irrigated rice. Plant Soil 218:1–10.
Ansari, R., S. S. M. Naqvi, A. N. Khanzada, and K. T. Hubick. 1998. Carbon-isotope discrimination in wheat under saline conditions. Pak. J. Bot. 30:87–93.
Condon, A. G., R. A. Richards, G. J. Rebetzke, and G. D. Farquhar. 2002. Improving intrinsic water-use efficiency and crop yield. Crop Sci. 42:122–131.
Farquhar, G. D. and J. Lloyd. 1993. Carbon and oxygen isotope effects in the exchange of carbon dioxide between terrestrial plants and the atmosphere. In Stable Carbon Isotopes and Plant Carbon-Water Relations. Eds. J. R. Ehleringer, A. E. Hall and J. D. Farquhar. Academic Press, London, pp 47–70.
Farquhar, G. D., J. R. Ehleringer, and K. T. Hubick. 1989. Carbon isotope discrimination and photosynthesis. Ann. Rev. Plant Physiol. Plant Mol. Biol. 40:503–537.
Gillion, J. S. and H. Griffiths. 1997. The influence of (photo)respiration on carbon isotope discrimination in plants. Plant Cell Env. 20:1217–1230.
Iyer, S. and A. Caplan. 1998. Products of proline catabolism can induce osmotically regulated genes in rice. Plant Physiol. 116:203–211.
Kalinkina, C. G. and T. M. Udel’nova. 1990. Effect of photorespiration on fractionation of stable carbon isotopes in a marine Chlorella. Fiziologiya Rastenii (Moscow) 37:96–104.
Loreto, F., D. Sebastiano, and G. Di Marco. 1999. Estimation of photorespiratory carbon dioxide recycling during photosynthesis. Austr. J. Plant Physiol. 26(8):733–736.
Loreto, F., M. Centrillo, and K. Chartzoulakis. 2003. Photosynthetic limitations in olive cultivars with different sensitivity to salt stress. Plant Cell Env. 26:595–608.
Lutts, S., J. M. Kinet, and J. Bouharmont. 1996. Effects of salt stress on growth, mineral nutrition and proline accumulation in relation to osmotic adjustment in rice (Oryza sativa L.) genotypes differing in salinity resistance. Plant Growth Reg. 19:207–218.
Menédez, C. M., and A. E. Hall. 1996. Heritability of carbon isotope discrimination and correlations with harvest index in cowpea. Crop Sci. 36:233–238.
Poss, J. A., S. R. Grattan, D. L. Suarez, and C. M. Grieve. 2000. Stable carbon isotope discrimination: an indicator of cumulative salinity and boron stress in Eucalyptus camaldulensis. Tree Physiol. 20:1121–1127.
Rebetzke, G. J., A. G. Condon, R. A. Richards, and G. D. Farquhar. 2002. Selection for reduced carbon isotope discrimination increases aerial biomass and grain yield of rainfed bread wheat. Crop Sci. 42(3): 739–745.
Roeske, C. A., O’Leary, M. H. 1984. Carbon isotope effects on the enzyme-catalyzed carboxylation of ribulose bisphosphate. Biochemistry. 23:6275–84.
Sanchez-Diaz, M., J. L. Garcia, M. C. Antolin, and J. L. Araus. 2002. Effects of soil drought and atmospheric humidity on yield, gas exchange, and stable carbon isotope composition of barley. Photosynthetica. 40:415–421.
SAS Institute, Inc. 1987. SAS/STAT user’s guide, version 6, 4th edition. SAS Institute, Inc., Cary, NC
Zeng, L. M. C. Shannon, and C. M. Grieve. 2002. Evaluation of salt tolerance in rice genotypes by multiple agronomic parameters. Euphytica 127:235–245
Zeng, L., J. A. Poss, C. Wilson, A. S. E. Draz, G. B. Gregorio, and C. M. Grieve. 2003. Evaluation of salt tolerance in rice genotypes by physiological characters. Euphytica 129:281–292.
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Poss, J.A., Zeng, L. & Grieve, C.M. Carbon isotope discrimination and salt tolerance of rice genotypes. CEREAL RESEARCH COMMUNICATIONS 32, 339–346 (2004). https://doi.org/10.1007/BF03543319
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DOI: https://doi.org/10.1007/BF03543319