Skip to main content
Log in

Rapid detection of staphylococcus saprophytius using primer specific PCR

  • Published:
Acta Biologica Hungarica Aims and scope Submit manuscript

Abstract

Staphylococcus saprophyticus is one of the most frequently encountered clinically significant members of the coagulase-negative staphylococci. A set of species-specific PCR primers was defined for the detection of Staphylococcus saprophyticus. These primers target variable regions (V3 and V6) of the 16S rRNA gene. Primer-specific PCR has potential applications in epidemiological studies and diagnosis of Staphylococcus saprophyticus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. DeLong, E. F., Wickham, G. S., Pace, N., Buyser, M. L., Morvan, A., Aubert, S., Dilasser, F., El Solh, N. (1992) Evaluation of a ribosomal RNA gene probe for the identification of species and subspecies within the genus Staphylococcus, J. Gen. Microbiol. 138, 889–899.

    Article  Google Scholar 

  2. Gillespie, W. A., Sellin, M. A., Gill, P., Stephens, M., Tuckwell, L. A., Hilton, A. L. (1978) Urinary tract infection in young women with special reference to Staphylococcus saprophyticus. J. Clin. Pathol. 31, 348–350.

    Article  CAS  Google Scholar 

  3. Golledge, C. L. (1988) Staphylococcus saprophyticus bacteremia. J. Infect. Dis. 157, 215.

    Article  CAS  Google Scholar 

  4. Gutell, R. R., Larsen, N., Woese, C. R. (1994) Lesson from an evolving rRNA: 16S 23S rRNA structures from a comparative perspective. Microbiol. Rev. 58, 10–26.

    CAS  PubMed  PubMed Central  Google Scholar 

  5. Hovelius, B., Mardh, P. A, (1974) Staphylococcus saprophyticus as a common cause of urinary tract infections. Rev. Infect. Dis. 6, 328–337.

    Article  Google Scholar 

  6. Jordan, P. A., Irvani, A., Richard, G. A., Baer, H. (1980) Urinary tract infections caused by Staphylococcus saprophyticus. J. Infect. Dis. 142, 510–515.

    Article  CAS  Google Scholar 

  7. Kloos, W, E., Bannerman, T, L, (1994) Update on clinical significance of coagulase-negative staphylococci. Clin. Microbiol. Rev. 7, 117–140.

    Article  CAS  Google Scholar 

  8. Kloos, W. E., Musselwhite, M. S, (1975) Distribution and persistence of Staphylococcus and Micrococcus species and other aerobic bacteria on human skin. Appl. Microbiol. 39, 195–207.

    Google Scholar 

  9. Kloos, W. E., Schleifer, K. H., Gotz, F. (1992) The genus Staphylococcus. In: The Prokaryotes: A Handbook on Habitats, Isolation and Identification of Bacteria. Springer-Verlag, Berlin—New York.

    Google Scholar 

  10. Ludwig, W., Schleifer, K. H. (1994) Bacterial phylogeny based on 16S and 23S rRNA sequences analysis. FEMS Microbiol. Rev. 15, 155–173.

    Article  CAS  Google Scholar 

  11. Marrie, T. J., Kwan, C., Noble, M. A., West, A., Duffield, L. (1982) Staphylococcus saprophyticus as cause of urinary tract infections. J. Clin. Microbiol. 16, 427–431.

    Article  CAS  Google Scholar 

  12. Rupp, M. E., Soper, D. E., Archer, G. L. (1992) Colonization of the female genital tract with Staphylocovvus saprophyticus. J. Clin. Microbiol. 30, 2975–2979.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Dedicated to the memory of Professor Gábor Szabó.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gaszewska-Mastalarz, A., Zakrzewska-Czerwinska, J. & Mordarski, M. Rapid detection of staphylococcus saprophytius using primer specific PCR. BIOLOGIA FUTURA 48, 319–322 (1997). https://doi.org/10.1007/BF03543203

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03543203

Keywords

Navigation