Abstract
The structures of two adenosine receptor ligands were determined from single-crystal X-ray diffraction analysis: DMX-7R, 1,3-dimethylxanthine-7-riboside monohydrate, monoclinic P21, a = 4.633(2), b = 6.755(1), c = 22.921(3) Å, β = 92.11(3)°, final R = 0.034 for 1441 observed reflections; CGS 15943, 9-chloro-2-(2-furyl)[1,2,4]triazolo[ 1.5-c]quinazolin-5-amine methane sulfonate, monoclinic, P21, a = 7.887(3), b = 10.014(2), c = 20.601(5) Å, β = 95.36(3)°, final R = 0.059 for 2150 observed reflections. DMX-7R, a partial A1 agonist, is a nucleoside which displays an anti conformation around the glycosidic bond and a 3T2 conformation of the ribose ring. Extensive hydrogen bonds are observed which include all the hydroxyl groups of the ribose, a water molecule and the sp2 hybridized N9 atom. CGS 15943 is a selective A2a antagonist. Its structure presents some analogies with that of adenine. The lack of the ribose ring is a necessary condition for antagonistic activity, while the furane substituent and the fused chloropheny1 ring act as modulators of the A2 selectivity. The molecule, roughly planar, is protonated at the imino nitrogen of the pyrimidine ring and forms strong hydrogen bonds with two oxygens of the methane sulfonate ion.
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Acknowledgments
The authors thank Dr. A.P. IJzerman (Centre for Bio-Pharmaceutical Sciences, Leiden, The Netherlands) for having provided the crystals of DMX-7R, Professor G. Gilli (University of Ferrara, Italy) for useful discussion, and the Italian Ministry for University and Scientific and Technological Research (MURST, Rome: 40% and 60% projects)
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Dalpiaz, A., Bertolasi, V., Ferretti, V. et al. Crystal and molecular structures of two adenosine receptor ligands: 1,3-dimethylxanthine-7-riboside monohydrate (DMX-7R), a partial A1 agonist, and 9-chloro-2-(furyl)[1,2,4]triazolo[1,5-c]quinazolin-5-amine methane sulfonate (CGS 15943), a selective A2a antagonist. J Chem Crystallogr 27, 59–65 (1997). https://doi.org/10.1007/BF03543088
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DOI: https://doi.org/10.1007/BF03543088