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Adaptation to host (chickpea) genotype by isolates of Didymella rabiei following repeated cycles of infection

Adaptierung an den Genotyp des Wirtes Kichererbse durch Isolate von Didymella rabiei infolge wiederholter Infektionszyklen

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Abstract

Ascochyta blight of chickpea (Cicer arietinum), caused by Didymella rabiei, is an important disease of chickpea world- wide. Under favourable conditions, the pathogen completes several disease cycles in one cropping season. To assess the effect of multiple infection cycles on the aggressiveness of D. rabiei, two isolates of the pathogen were inoculated onto each of three chickpea cultivars, then re-isolated and re-inoc- ulated onto the same cultivar for five generations. Subcultures of each isolate were retained after each generation, and the aggressiveness of each subculture was assessed at the end of the study by measuring blight severity when re-inoculated onto all three chickpea cultivars. The effect of isolate genera- tion was small but significant. Blight severity on a cultivar increased with repeated culturing on that cultivar. This indi- cates that the aggressiveness of the pathogen population in the field is likely to increase on a cultivar as a result of repeat- ed infection of that cultivar. Thus, avoiding repeated cultiva- tion of a cultivar in a field may reduce the potential severity of Ascochyta blight outbreaks.

Zusammenfassung

Der durch den pflanzenpathogenen Pilz Didymella rabiei verursachte Ascochyta-Brand der Kichererbse (Cicer arietinum) ist weltweit eine der bedeutendsten Erkrankungen dieser Nutzpflanze. Unter günstigen Bedingungen vollendet der Erreger mehrere Entwicklungszyklen pro Vegetationsperiode. Zur Untersuchung des Einflusses wiederholter Infektionszyklen auf die Agressivität des Pathogens wurden drei Kichererbsensorten mit je zwei Isolaten von D. rabiei inokuliert, der Erreger reisoliert und dieselben Sorten für weitere fünf Erregerzyklen inokuliert. Nach jedem Zyklus wurden die Isolate subkultiviert und ihre Aggressivität durch Erhebung der Befallsstärke nach Reinokulation aller drei Sorten gemessen. Der Einfluss wiederholter Zyklen war gering aber signifikant. Die Befallsstärke stieg mit wiederholter Kultivierung der Isolate bei allen Sorten, was darauf hindeutet, dass die Aggressivität einer Erregerpopulation mit wiederholter Infektion einer Kichererbsensorte im Feld zunimmt. Die Vermeidung zu enger Fruchtfolgen könnte daher die Stärke von Epidemien des Ascochyta-Brands der Kichererbse vermindern.

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References

  • Ahmed, H.U., C.C. Mundt, M.E. Hoffer, S.M. Coakley, 1996: Selective influence of wheat cultivars on pathogenicity of Mycosphaerella graminicola (anamorph Septoria tritici). Phytopathology 86, 454–458.

    Article  Google Scholar 

  • Ahmed, H.U., K.F. Chang, S.F. Hwang, R.J. Howard, 2005: Surveillance of ascochyta blight of chickpea in southern Alberta in 2004: Can. J. Plant Pathol. 27, 145.

    Google Scholar 

  • Ahmed, H.U., K.F. Chang, S.F. Hwang, B.D. Gossen, R.J. Howard, T. D. Warkentin, 2006: Components of disease resistance in desi and kabuli chickpea varieties against ascochyta blight. Plant Pathol. J. 5, 336–342.

    Article  Google Scholar 

  • Ahmed, H.U., K.F. Chang, S.F. Hwang, S.E. Strelkov, D.J. Bing, G.D. Turnbull, 2007: Pathogenic diversity of Didymella rabiei isolates from southern Alberta, Canada. J. Plant Dis. Protect. 114, 189–195.

    Google Scholar 

  • Armstrong, C.L., G. Chongo, B.D. Gossen, L.J. Duczek, 2001: Mating type distribution and incidence of teleomorph of Ascochyta rabiei (Didymella rabiei) in Canada. Can. J. Plant. Pathol. 23, 110–113.

    Article  Google Scholar 

  • Chang, K. F., R.J. Howard, M.A. Briant, D.A. Burke, M. Clawson, 2000: Survey for ascochyta blight and root rot diseases of chickpea in southern Alberta in 1999: Can. Plant Dis. Surv. 80, 83–85.

    Google Scholar 

  • Chang, K.F., H.U. Ahmed, S.F. Hwang, R.J. Howard, 2005: Manipulation of row spacing and seeding rate for the management of ascochyta blight of chickpea. Can. J. Plant Pathol. 27, 145–146.

    Article  Google Scholar 

  • Chang, K.F., S.F. Hwang, A.H. Khadhair, H.U. Ahmed, S.E. Strelkov, 2008: Molecular diversity of Ascochyta rabiei from chickpea in Alberta, Canada. Plant Pathol. J. 7, 20–26.

    Article  CAS  Google Scholar 

  • Chen, W., C.J. Coyne, T.L. Peever, F.J. Muehlbauer, 2004: Characterization of chickpea differentials for pathogenicity assay of ascochyta blight and identification of chickpea accessions resistant to Didymella rabiei. Plant Pathol. 53, 759–769.

    Article  Google Scholar 

  • Chongo, G., B.D. Gossen, 2003: Diseases of chickpea. In: K.L. Bailey, B.D. Gossen, R. Gugel, R.A.A. Morrall (eds.): Diseases of Field Crops in Canada, pp. 185–190. Canadian Phytopathological Society, Saskatoon, SK, Canada.

    Google Scholar 

  • Chongo, G., B.D. Gossen, L. Buchwaldt, T. Adhikari, R.R. Rimmer, 2004: Genetic diversity of Ascochyta rabiei in Canada. Plant Dis. 88, 4–10.

    Article  Google Scholar 

  • Gan, Y., P. Liu, C. Mcdonald, 2002: Severity of ascochyta blight in relation to leaf type in chickpea. Crop Sci. 43, 2291–2294.

    Article  Google Scholar 

  • Hall, T.A., 1999: BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp. Ser. 41, 95–98.

    CAS  Google Scholar 

  • Harrower, K.M., 1977: Specialization of Leptosphaeria nodorum to alternative graminicious hosts. Trans. Br. Mycol. Soc. 65, 101–103.

    Article  Google Scholar 

  • Horsfall, J.G., R.W. Barratt, 1945: An improved grading system for measuring plant diseases. Phytopathology 35, 65.

    Google Scholar 

  • Jan, H., M.V. Wiese, 1991: Virulence forms of Ascochyta rabiei affecting chickpea in Palouse. Plant Dis. 75, 904–906.

    Article  Google Scholar 

  • King, J.E., R.J. Cook, S.C. Melville, 1983: A review of septoria disease of wheat and barley. Ann. Appl. Biol. 103, 345–375.

    Article  Google Scholar 

  • Mcvicar, R., P. Pearse, K. Panchuk, C. Brenzil, S. Hartley, C. Harris, J. Yasinowski, D. Goodwillie, T. Warkentin, S. Banniza, 2006: Chickpea in Saskatchewan. Saskatchewan Agriculture and Food Factsheet. [Online] Available: http://www.agr.gov.sk.ca/docs/crops/pulses/production_infor-mation/chickpea2002.asp?firstPick=Crops&secondpick=Pulses&thirdpick=Production%20Information. Accessed August, 21, 2006.

    Google Scholar 

  • Navas-Cortés, J.A., E. Pérez-Artés, R.M. Jiménez-Díaz, A. Llobell, B.W. Brainbridge, J.B. Heale, 1998: Mating type, pathotype, RAPDs analysis in Didymella rabiei, the agent of ascochyta blight of chickpea. Phytoparasitica 26, 199–212.

    Article  Google Scholar 

  • Nene, Y.L., 1982: A review of ascochyta blight of chickpea. Trop. Pest. Manage. 28, 61–70.

    Article  Google Scholar 

  • Nene, Y.L., M.V. Reddy, 1987: Chickpea diseases and their control. In: M.C. Saxena, K.B. Singh (eds.): The Chickpea, pp. 233–270. Commonwealth Agricultural Bureaux International, Oxon, UK.

    Google Scholar 

  • Osbourn, A.E., R. Scott, C.E. Caten, 1986: The effect of host passaging on the adaptation of Septoria nodorum to wheat or barley. Plant Pathol. 35, 135–145.

    Article  Google Scholar 

  • Reddy, M.V., S. Kabbabeh, 1985: Pathogenic variability in Ascochyta rabiei (Pass) Lab. in Syria and Lebanon. Phytopathol. Medit. 24, 265–266.

    Google Scholar 

  • Rufty, R.C., T.T. Herbert, C.F. Murphy, 1981: Variation in virulence in isolates of Septoria nodorum. Phytopathology 71, 593–596.

    Article  Google Scholar 

  • Sas Institute Inc., 2005: SAS OnlineDoc® 9.1.3. SAS Institute Inc. Cary, N.C. [Online] Available: http://www.v8doc.sas.com/sashtml/ [2005 Aug. 22].

    Google Scholar 

  • Singh, G., 1990: Identification and designation of physiological races of Ascochyta rabiei in India. Ind. Phytopathol. 43, 48–52.

    Google Scholar 

  • Singh, K.B., M.V. Reddy, Y.L. Nene, 1984: International testing of chickpeas for resistance to ascochyta blight. Plant Dis. 68, 782–784.

    Article  Google Scholar 

  • Thompson, J.D., T.J. Gibson, F. Plewniak, F. Jeanmougin, D.G. Higgins, 1997: The Clustal_X Windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucl. Acids Res. 24, 7876–4882.

    Google Scholar 

  • Vail, S., S. Banniza, 2008: Structure and pathogenic variability in Ascochyta rabiei populations on chickpea in the Canadian prairies. Plant Pathol. 57, 665–673.

    Article  Google Scholar 

  • Vir, S., J.S. Grewal, 1974: Physiological specialization in Ascochyta rabiei, the causal organism of gram blight. Ind. Phytopathol. 27, 265–266.

    Google Scholar 

  • White, T.J., T. Bruns, S. Lee, J. Taylor, 1990: Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: M.A. Innis, D.H. Gelfand, J.J. Sninsky, T.J. White (eds.): PCR Protocols, A Guide to Methods and Applications, pp. 315–322. Academic Press, San Diego, CA, USA.

    Google Scholar 

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Correspondence to S. F. Hwang.

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Ahmed, H.U., Hwang, S.F., Gossen, B.D. et al. Adaptation to host (chickpea) genotype by isolates of Didymella rabiei following repeated cycles of infection. J Plant Dis Prot 116, 177–181 (2009). https://doi.org/10.1007/BF03356307

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