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Quercetin influences response in Nicotiana megalosiphon infected by satellite-associated cucumber mosaic virus

Quercetin beeinflusst die Reaktion von Nicotiana magalosiphon gegenüber Satelliten-assoziierten Gurkenmosaikvirus-Infektionen

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Abstract

Quercetin is a prominent flavonoid with a remarkable spectrum of biochemical activities. Its impact on human health, as well as its role in the normal development of plants has been exten-sively studied. In this work the influence of quercetin on satel-lite associated cucumber mosaic virus (CMV) infection and the response of Nicotiana megalosiphon host plants were investigated. Daily continuous flavonoid treatment of infected plants resulted in a significant increase in leaf mass and total protein synthesis. Attenuation of the yellowing symptom was also recorded, accompanied by lower expression of both viral and satellite replicative dsRna. This correlated with the decreased total peroxidase activity established in those plants. Expression of some glycoproteins (38 and 22.5 kDa), absent in healthy plants, were suppressed when infected plants were treated with quercetin. At the same time, some other infection-specific glycoproteins (22, 24 and 29 kDa) were significantly amplified by quercetin. The inability of quercetin to bind Cmv nucleoprotein or isolated ssRna was demonstrated. Quercetin has not shown any effect on Hsp70 synthesis in Cmv-infected plants.

Zusammenfassung

Quercetin ist ein bedeutendes Flavonoid mit einem bemer-kenswerten Spektrum biochemischer Aktivitäten. Seine Bedeutung für die menschliche Gesundheit, wie auch seine Funktion in der normalen pflanzlichen Entwicklung wurden weitgehend untersucht. In dieser Arbeit wurde der Einfluss von Quercetin auf die Abwehrantwort von Nicotiana megalo-siphon-Wirtspflanzen gegenüber Satelliten-assoziierten Gur-kenmosaikvirus (CMV)-Infektionen untersucht. Tägliche kon-tinuierliche Flavonoidbehandlung von infizierten Pflanzen resultierte in einem signifikanten Anstieg der Blattmasse und der gesamten Proteinsynthese. Eine Abschwächung der Ver-gilbungssymptome wurde ebenfalls verzeichnet, begleitet von geringerer Expression von replikativer Virus- und Satelliten-RNA. Dies korrelierte mit der Abnahme der Gesamtperoxidase-aktivität, die für diese Pflanzen nachgewiesen wurde. Einige Glycoproteine (38 und 22,5 kDa), die in gesunden Pflanzen nicht vorkommen, wurden unterdrückt, wenn die Pflanzen mit Quercetin behandelt wurden. Zur gleichen Zeit wurden einige andere infektionsspezifische Glycoproteine (22, 24 und 29 kDa) signifikant durch Quercetin verstärkt. Die Unfähigkeit von Quercetin, das CMV-Nukleokapsid oder isolierte ssRNA zu binden, wurde nachgewiesen. Quercetin zeigte keinen Effekt auf die HSP70-Synthese bei CMV-infizierten Pflanzen.

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Authors and Affiliations

  1. Department of Biology, Faculty of Science, University of Zagreb, Rooseveltov trg 6, 10000, Zagreb, Croatia

    Gordana Rusak, Mladen Krajačićl & Marijana Krsnik-Rasol

  2. Institut für Zoologie, Technische Universität Dresden, 01062 Dresden, Germany

    Herwig O. Gutzeit

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  1. Gordana Rusak
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  2. Mladen Krajačićl
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  3. Marijana Krsnik-Rasol
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  4. Herwig O. Gutzeit
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Correspondence to Gordana Rusak.

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Rusak, G., Krajačićl, M., Krsnik-Rasol, M. et al. Quercetin influences response in Nicotiana megalosiphon infected by satellite-associated cucumber mosaic virus. J Plant Dis Prot 114, 145–150 (2007). https://doi.org/10.1007/BF03356210

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