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Effects of vitamin D3metabolites on thyrotropin secretion from rat pituitary cells in culture

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Abstract

The effects of several vitamin D3 metabolites on TSH secretion were investigated using rat pituitary cells in culture. After a 24-h incubation in Dulbecco’s medium, only 25,26-dihydroxyvitamin D3 [25,26(OH)2D3] at 10-6 M increased the basal TSH secretion (36% above control). The TRH-induced TSH secretion increased dose-dependently with increasing concentrations of 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] showing a significant increase at 10-8 M (41% above control). At 10-5 M 25,26(OH)2D3 the TRH-stimulated TSH secretion was also increased (63% above control). In medium containing charcoal-treated serum, 1, 24,25-trihydroxyvitamin D3 [1,24,25(OH)3D3] decreased the TRH-induced TSH secretion dose dependency (34% below control at 10-8 M), while the effects of both 1,25(OH)2D3 and 25,26(OH)2D3 were blunted. Our results indicate that several vitamin D3 metabolites may affect pituitary TSH secretion.

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Authors and Affiliations

  1. Endocrine Research Laboratory, University of Helsinki, Minerva Foundation, Institute tor Medical Research, P.O. Box 819, 00101, Helsinki, Finland

    K. Törnquist, L. Forss & C. Lamberg-Allardt

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  1. K. Törnquist
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  2. L. Forss
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  3. C. Lamberg-Allardt
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Törnquist, K., Forss, L. & Lamberg-Allardt, C. Effects of vitamin D3metabolites on thyrotropin secretion from rat pituitary cells in culture. J Endocrinol Invest 13, 391–395 (1990). https://doi.org/10.1007/BF03350686

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