Abstract
A radioimmunoassay for plasma arginine vasopressin (AVP) has been developed based on R 2 antibody of Thomas and Lee, synthetic standard (Ferring) and extraction on Sep-Pak column. High recovery of AVP (~79%) was achieved with a detection limit of 0.25 pg/ml. By improving the technique of measurement of plasma osmolality an intraassay coefficient of variation less than 1% was obtained. Physiological studies performed with this method demonstrated that AVP becomes undetectable after water loading 20 ml per Kg of water po; (N = 6) and increases in response to hypertonic saline infusion (0.05 ml/kg/min; N = 15) with a linear relationship between plasma osmolality and AVP in individual subjects; this relationship is maintained when the test is repeated in the same subjects. However when pooling all data together, the relationship between plasma osmolality and AVP is best expressed by an exponential relationship. This implies that after AVP release is initiated, the concentration of the hormone increases more rapily than plasma osmolality and the release is continuous possibly due to recruitment of increasing number of neuronal units whose osmotic threshold varies from individual to individual.
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References
Zerbe R.L., Robertson G.L. A comparison of plasma vasopressin measurements with a standard indirect test in the differential diagnosis of polyuria. N.Engl. J. Med. 305: 1539, 1981.
Bichet D., Szatalowicz V., Chaimovitz C., Schrier R.W. Role of vasopressin in the abnormal water excretion in cirrhotic patients. Ann. Intern. Med. 96: 413, 1982.
Robertson G.L. Athar S. The interaction of blood osmolality and blood volume in regulating plasma vasopressin in man. J. Clin. Endocrinol. Metab. 42: 613, 1976.
Robertson G.L. The regulation of vasopressin function in health and disease. Recent Prog. Horm. Res. 33: 333, 1977.
Shimamoto K., Murase T. Yamaji T. A heterologous radioimmunoassay for argin vasopressin. J. Lab. Clin. Med. 87: 338, 1976.
Thomas T.N., Lee M.R. The specificity of antisera for the radioimmunoassay of arginine vasopressin in human plasma and urine during water loading and dehydration. Clin. Sci. Mol. Med. 51: 525, 1976.
Redetzki H.M., Hughes J.R., Redetzki J.E. Differences between serum and plasma osmolalities and their relationship to lactic acid values. Proc. Soc. Exp. Biol. Med. 139: 315, 1972.
Greenwood F.C., Hunter W.M., Glover J.S. The preparation of 131 I-Iabelled human growth hormone of high specific radioactivity. Biochem. J. 89: 114, 1963.
La Rachelle FT., North W.G., Stern P. A new extraction of arginine vasopressin from blood; the use of octadeca silylsilica. Pfluegers Arch. 387: 79, 1980.
Draper N.R., Smith H. Applied regression analysis. Wiley, New York, 1966.
Möhring J. Ferring workshop on the radioimmunoassay of vasopressin. Endokrinologie 6: 201, 1978.
Robertson G.L., Athar S., Shelton R.L. Osmotic control of vasopressin function. In: Andreoli T.E., Grantham J.J., Floyd CR. jr. (Eds.) Disturbances in body fluid osmolality. The Williams and Wilkins Co., Baltimore, 1977, p. 125.
Weitzman R.E., Fisher D.A. Log linear relationship between plasma arginine vasopressin and plasma osmolality. Am. J. Physiol. 233: E37, 1977.
Thibonnier M., Sassano P., Daufresne S., Corvol P., Menard J. Osmoregulation and renal effects of vasopressin in normal and mildly hypertensive subjects. Kidney Int. 25: 411, 1984.
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Bevilacqua, M., Meroni, R., Dagani, R. et al. Role of blood osmolality in the regulation of vasopressin secretion in man: application of a new radioimmunoassay method for vasopressin. J Endocrinol Invest 8, 97–101 (1985). https://doi.org/10.1007/BF03350655
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DOI: https://doi.org/10.1007/BF03350655