Abstract
Islets of Langerhans from ob/ob mice were microdissected and stored for one week at 8 C or 37 C in a high-potassium “intracellular medium” or in a traditional culture medium (RPMI 1640 + fetal calf serum). Subsequent glucose-stimulated insulin release and islet morphology were best preserved by 8 C-storage in the high-potassium “intracellular medium”. This suggests that normal, adult, mammalian tissues which do not readily divide in vitro may be better preserved for short periods of time at refrigerator temperatures in a high-potassium medium than by conventional culture at 37 C in a tissue culture medium.
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Frankel, B.J., Norlund, N.R. Cold storage (8 C) of islets of Langerhans in a high-potassium “intracellular medium”: near-normal insulin release and morphology after one week. J Endocrinol Invest 8, 59–62 (1985). https://doi.org/10.1007/BF03350642
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DOI: https://doi.org/10.1007/BF03350642