Abstract
An immunohistochemical study with two rabbit polyclonal antibodies I-AR76 and CA-08-351 against Endothelin-1 (ET-1) was performed in 133 human thyroid specimens: 5 normal thyroids, 30 multinodular goiters (15 toxic and 15 nontoxic), 20 Graves’ diseases, 5 Hashimoto’s thyroiditis, 26 adenomas (6 Hürthle cell, 16 toxic and 4 nontoxic), 30 classic papillary carcinomas, 3 minimally invasive follicular carcinomas, 1 widely invasive follicular carcinoma, 3 undifferentiated carcinomas and 10 medullary carcinoma. All normal thyroids, non toxic multinodular goiters and non toxic adenomas, 4 (66%) Hürthle cell adenomas, 3 (15%) Graves’ diseases, 1 (33%) case of minimally invasive follicular carcinoma showed rare follicular cells with weak cytoplasmic immunoreactivity. Many immunoreactive follicular cells, with or without oxyphilic changes, were observed in all specimens of Hashimoto’s disease, while the lymphocytic infiltrate was always negative. Twenty-seven (90%) classic papillary carcinomas were positive. Immunoreactivity was intra-cytoplasmic, weak in 14 cases and intense in 13. The cells of toxic adenoma and toxic multinodular goiter were negative, whereas the acellular stroma was intensely positive in both cases. Medullary and undifferentiated carcinomas were negative. These results show ET-1 immunoreactivity in normal and pathological human thyroids. In particular, the high content of this peptide in the thyroid papillary carcinoma suggests that ET-1, whose mitogenic role has recently been emphasized, could be involved in the growth of this tumor.
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Yanagisawa M., Kurihara H., Kimura S., Tomobe Y., Kobayashi M., Mitsui Y., Yazaki Y., Goto K., Masaki T. A novel potent vasoconstrictor peptide produced by vascular endothelial cells. Nature 332: 411, 1988.
Rozengurt N., Springall D.R., Polak J.M. Localization of endothelin-like immunoreactivity in airway epithelium of rats and mice. J. Pathol. 160: 5, 1990.
Kitamura K., Tanaka T., Kato J., Eto T., Tanaka K. Regional distribution of immunoreactive endothelin in porcine tissue: abundance in inner medulla of kidney. Biochem. Biophys. Res. Commun. 161: 348, 1989.
Giaid A., Gibson S.J., Ibrahim N.B.N., Legon S., Bloom S.R., Yanagisawa M., Masaki T., Varndell I.M., Polak J.M. Endothelin-1 an endothelium-derived peptide is expressed in neurons of the human spinal cord and dorsal root ganglia. Proc. Natl. Acad. Sci. USA 86: 7634, 1989.
Inoue A., Yanagisawa M., Kimura S., Kasuya Y., Miyauchi T., Goto K., Masaki T. The human endothelin family: three structurally and pharmacologically distinct isopeptides predicted by three separate genes. Proc. Natl. Acad. Sci. USA 86: 2863, 1989.
Rubanyi G.M., Parker Botelho L.H. Endothelins. FASEB J. 5: 2713, 1991.
Simonson M.S., Dunn M.J. Cellular signaling by peptides of the endothelin gene family. FASEB J. 4: 2989, 1990.
Shichiri M., Hirata Y., Nakajima T., Ando K., Imai T., Yanagisawa M., Masaki T., Marumo F. Endothelin-1 is an autocrine/paracrine growth factor for human cancer cell lines. J. Clin. Invest. 87: 1867, 1991.
Colin I., Berbinschi A., Denef J.F., Ketelslegers J.M. Detection and identification of Endothelin-1 immuno-reactivity in rat and porcine thyroid follicular cells. Endocrinology 130: 544, 1992.
Miyakawa M., Tsushima T., Isozaki O., Demura H., Shizume K., Arai M. Endothelin-1 stimulates c-fos mRNA expression and acts as modulator on cell proliferation of rat FRTL5 thyroid cells. Biochem. Biophys. Res. Commun. 184: 231, 1992.
Eguchi K., Kawakami A., Nakashima M., Ida H., Sakito S., Sakai M., Terada K., Kawabe Y., Fukuda T., Ishikawa N., Ito K., Nagataki S. Stimulation of mitogenesis in human thyroid epithelial cells by endothelin. Acta Endocrinol. (Copenh.) 128: 215, 1993.
Laurent E., Mockel J., Van Sande J., Graff I., Dumont J.E. Dual activation by thyrotrophin of the phospholipase C and cyclic AMP cascades in human thyroid. Mol. Cell. Endocrinol. 52: 273, 1987.
Weiss S.J., Philp N.J., Grollman E.F. Effect of thyrotropin on iodide efflux in FRTL-5 cells mediated by Ca++. Endocrinology 114: 1108, 1984.
Marcocci C., Luini A., Santisteban P., Grollman E.F. Norepinephrine and thyrotropin stimulation of iodide efflux in FRTL-5 thyroid cells involves metabolites of arachidonic acid and is associated with iodination of thyroglobulin. Endocrinology 120: 1127, 1987.
Tseng Y.C., Lahiri S., Jackson S., Burman K.D., Wartofsky L. Endothelin binding to receptor and endothelin production by human thyroid follicular cells: effects of transforming growth factor-beta and thyrotropin. J. Clin. Endocrinol. Metab. 76: 156, 1993.
Jackson S., Tseng Y.C., Lahiri S., Burman K.D., Wartofsky L. Receptors for endothelin in cultured human thyroid cells and inhibition by endothelin of thyroglobulin secretion. J. Clin. Endocrinol. Metab. 75: 388, 1992.
Yoshizumi M., Kurihara H., Morita T., Yamashita T., Oh-hashi Y., Sugiyama T., Takaku F., Yanagisawa M., Masaku T., Yazaki Y. Interleukin-1 increases the production of endothelin-1 by cultured endothelial cells. Biochem. Biophys. Res. Commun. 166: 324, 1990.
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Lenziardi, M., Viacava, P., Fiorini, I. et al. Presence of endothelin-1 in the normal and pathological human thyroid. J Endocrinol Invest 18, 336–340 (1995). https://doi.org/10.1007/BF03347834
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DOI: https://doi.org/10.1007/BF03347834