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Prevalence of anti-thyroid peroxidase antibodies in autoimmune and nonautoimmune thyroid disorders in a relatively low-iodine environment

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Abstract

We evaluated the prevalence of antithyroid peroxidase antibodies (anti-TPO Ab) in 402 patients with thyroid disease and 30 healthy controls by a commercial radioimmunoassay (RIA) and compared the results with the passive hemagglutination (HA) method. The patients in the study had autoimmune thyroid disorders (AITD) such as Graves’ disease and Hashimoto’s disease or had nonautoimmune thyroid diseases (NAITD) such as thyroid cancer, congenital goiter, endemic goiter, and nodular goiter. Subjects were recruited from a population with a mild iodine deficiency (Sao Paulo, Brazil). The effect of specific therapy (for either thyrotoxicosis or chronic thyroiditis) on the circulating anti-TPO levels was also investigated. Positive anti-TPO Ab was detected in 89.9% of the patients with AITD as compared with a prevalence of positive tests of only 4.8% in patients with NAITD. Positive microsomal antibody (M Ab) was found in 68.4% of the patients with AITD and in 6.4% of the patients with NAITD. A positive and significant correlation was obtained between M Ab and anti-TPO Ab. A positive anti-TPO test with negative anti-M was found in 14.1% of the patients with AITD but in only 4.3% of the patients with NAITD and normal controls. These results suggest that anti-TPO Ab by RIA is more sensitive and specific than M Ab by HA. In patients with AITD, anti-TPO Ab levels usually decreased after treatment, suggesting that this parameter could be used in the follow-up of these thyroid disorders.

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References

  1. Medeiros-Neto G.A. Towards the eradication of iodine deficiency disorders in Brazil through a salt iodination program. Bull. World Health Org. 66: 637, 1988.

    CAS  PubMed Central  PubMed  Google Scholar 

  2. Doniach D. Humoral and genetic aspects of thyroid autoimmunity. Clin. Endocrinol. Metab. 4: 267, 1975.

    Article  CAS  Google Scholar 

  3. Khoury E.L., Hammond L., Bottazzo G.F., Doniach D. Presence of organ-specific “microsomal” auto-antigen on the surface of human thyroid cells in culture: its involvement in complement mediated cytotoxicity. Clin. Exp. Immunol. 45: 316, 1981.

    CAS  PubMed Central  PubMed  Google Scholar 

  4. Weetman A.P., MacGregor A.M. Autoimmune thyroid disease: developments in our understanding. Endocr. Rev. 5: 309, 1984.

    Article  CAS  PubMed  Google Scholar 

  5. Khoury E.L., Bottazzo G.F., Roitt I.M. The thyroid microsomal antibody revisited: its paradoxical binding in vitro to the apical surface of the follicular epithelium. J. Exp. Med. 159: 577, 1984.

    Article  CAS  PubMed  Google Scholar 

  6. Amino N., Hagan S.R., Yamada N., Refetoff S. Measurement of circulating thyroid microsomal antibodies by the tanned red cell hemagglutination technique: its usefulness in the diagnosis of autoimmune thyroid diseases. Clin. Endocrinol. (Oxf.) 5: 115, 1976.

    Article  CAS  Google Scholar 

  7. Cayzer I., Chalmers S.D., Doniach D., Swam G. An evaluation of two new hemagglutination tests for the rapid diagnosis of autoimmune thyroid disease. J. Clin. Pathol. 31: 1147, 1978.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  8. Schardt C.W., McLachlan S.M., Matheson J., Smith B.R. An enzyme-linked immunoassay for thyroid microsomal antibodies. J. Immunol. Meth. 55: 155, 1982.

    Article  CAS  Google Scholar 

  9. Pavlin K., Gaberscek S., Avcin J., Hojker S., Fidler V., Porenta M. Comparison of radioimmune methods for determining autoantibodies to thyroid peroxidase and microsomal antigen as well as the passive hemagglutination method. Period. Biol. 93: 443, 1991.

    Google Scholar 

  10. Czarnocka B., Ruf J., Ferrand M., Carayon P., Lissitszky S. Purification of the human thyroid peroxidase and its identification as the microsomal antigen involved in autoimmune thyroid diseases. Federation of European Biochemical Societies, vol. 190, Elsevier Science Publishers. Amsterdam, 1985, p. 147.

    Google Scholar 

  11. Seto P., Hirayu H., Magnuson R.P. Isolation of a complementary DNA clone for thyroid microsomal antigen. Homology with the gene for thyroid peroxidase. J.Clin. Invest. 80: 1205, 1987.

    CAS  Google Scholar 

  12. Ruf J., Czarnocka B., Ferrand M. Novel routine assay of thyroperoxidase auto-antibodies. Clin. Chem. 34: 2231, 1988.

    CAS  PubMed  Google Scholar 

  13. Mariotti S., Caturegli P., Piccolo G. Anti-thyroid peroxidase auto-antibodies in thyroid diseases. J. Clin. Endocrinol. Metab. 71: 661, 1990.

    Article  CAS  PubMed  Google Scholar 

  14. Doullay F., Ruf J., Carayon P., Codaccioni J.L. Autoantibodies to thyroperoxidase in various thyroid and autoimmune diseases. In: Carayon P., Ruf J. (Eds). Thyroperoxidase and Thyroid Autoimmunity. Colloque Inserm, vol. 207. John Libbey Eurotext Ltd, Paris. 1991, p. 285.

    Google Scholar 

  15. Bonnet H., Madec A.M., Rodien P., Latter R., Haond P., Allanic H., Orgiazzi J. Evaluation of anti-TPO antibody determination in various clinical situations. In: Carayon P., Ruf J. (Eds) Thyroperoxidase and Thyroid Autoimmunity. Colloque Inserm, vol. 207. John Libbey Eurotext Ltd., Paris. 1990, p. 315.

    Google Scholar 

  16. Feldt-Rasmussen U. Anti-thyroid peroxidase antibodies in thyroid disorders and non-thyroid autoimmune diseases. Autoimmunity 9: 245, 1991.

    Article  CAS  PubMed  Google Scholar 

  17. Hegedus L., Hansen J.M., Feldt-Rasmussen U., Hansen B.M., HierMadsen M. Influence of thyroxine treatment on thyroid size and antithyroid peroxidase antibodies in Hashimoto’s thyroiditis. Clin. Endocrinol. 35: 235, 1991.

    Article  CAS  Google Scholar 

  18. Boukis N.M.A., Koutras D.A., Souvatzoglou A., Evangelopoulou A., Vrontakis M., Moulopoulos S.M. Thyroid hormone and immunological studies in endemic goiter. J. Clin. Endocrinol. Metab. 57: 856, 1983.

    Google Scholar 

  19. Harach H.R., Escalante D.A., Onativia A., Lederer-Outes J., Saravia-Day E., Williams E.D. Thyroid carcinoma and thyroiditis in an endemic goitre region before and after iodine prophylaxis. Acta Endocrinol. (Copenh.) 108: 55, 1985.

    CAS  Google Scholar 

  20. Knobel M., Medeiros-Neto G. Iodized oil treatment for endemic goiter does not induce the surge of positive serum concentration of antithyroglobulin or anti-microsomal antibodies. J. Endocrinol. Invest. 9: 321, 1986.

    Article  CAS  PubMed  Google Scholar 

  21. Prentice L.M., Phillips D.I.W., Sarsero D., Beever K., McLachlan S.M., Smith B.R. Geographical distribution of subclinical autoimmune thyroid disease in Britain: A study using highly sensitive direct assays for autoantibodies to thyroglobulin and thyroid peroxidase. Acta Endocrinol. (Copenh) 123: 493, 1990.

    CAS  Google Scholar 

  22. Roti E., Minelli R., Gardini E., Bianconi L., Neri T., Gavaruzzi G., Ugolotti G., Salvo D., Braverman L.E. Impaired intrathyroidal iodine organification and iodine-induced hypothyroidism in euthyroid women with a previous episode of postpartum thyroiditis. J. Clin. Endocrinol. Metab. 73: 958, 1991.

    Article  CAS  PubMed  Google Scholar 

  23. Bogner V., Schleusener H., Wall J.R. Antibody dependent cell mediated cytotoxicity against human thyroid cells in Hashimoto’s thyroiditis but not Graves’ disease. J. Clin. Endocrinol. Metab. 59: 734, 1984.

    Article  CAS  PubMed  Google Scholar 

  24. Wadeleux P., Winand-Devigne J., Ruf J., Carayon P., Winard R. Cytotoxic assay of circulating thyroid peroxidase antibodies. Autoimmunity 4: 247, 1989.

    Article  CAS  PubMed  Google Scholar 

  25. Väkevä A., Kontiainen S., Miettinen A., Schleaska A., Maenpaa J. Thyroid peroxidase antibodies in children with autoimmune thyroiditis. J.Clin. Pathol. 45: 106, 1992.

    Article  PubMed Central  PubMed  Google Scholar 

  26. Roti E., Gardini E., Minelli R., Bianconi L., Braverman L.E. Prevalence of anti-thyroid peroxidase antibodies in serum in the elderly: comparison with other tests for antithyroid antibodies. Clin. Chem. 38: 88, 1992.

    CAS  PubMed  Google Scholar 

  27. Kohno Y., Hiyama Y., Shimazo N., Niimi H., Nakajima H., Hosoya T. Autoantibodies to thyroid peroxidase in patients with chronic thyroiditis: effect of antibody binding on enzyme activities. Clin. Exp. Immunol. 65: 534, 1986.

    CAS  PubMed Central  PubMed  Google Scholar 

  28. DeGroot L.J. Heterogeneity of human autoantibodies to TPO. In: Carayon P., Ruf J. (Eds). Thyroperoxidase and Thyroid Autoimmunity. Colloque Inserm, vol. 207. John Libbey Eurotext Ltd., Paris, 1990, p. 177.

    Google Scholar 

  29. Chiovato L., Marcocci C., Mariotti S., Mori A., Pinchera A. L-thyroxine therapy induces a fall of thyroid microsomal and thyroglobulin antibodies in idiopathic myxedema and in hypothyroid, but not in euthyroid Hashimoto’s thyroiditis. J. Endocrinol. Invest. 9: 299, 1986.

    Article  CAS  PubMed  Google Scholar 

  30. Azizi F. Environmental iodine intake affects the response to methimazole in patients with diffuse toxic goiter. J. Clin. Endocrinol. Metab. 61: 374, 1985.

    Article  CAS  PubMed  Google Scholar 

  31. Reinwein D., Benker G., Koning M.P., Pinchera A., Schatz H., Schleusener H. The different types of hyperthyroidism in Europe. Results of a prospective survey of 924 patients. J. Endocrinol. Invest. 11: 193, 1988.

    CAS  Google Scholar 

  32. Kohno Y., Maito N., Hiyama Y., Shimazo N., Suzuki N., Tarutani O., Niimi H., Nakajima H., Hosoya T. Thyroglobulin and thyroid peroxidase share common epitopes recognized by autoantibodies in patients with chronic autoimmune thyroiditis. J. Clin. Endocrinol. Metab. 67: 899, 1988.

    Article  CAS  PubMed  Google Scholar 

  33. Ruf J., Ferrand M., Durand-Gorde J.-M., Carayon P. Immunopurification and characterization of thyroid autoantibodies with dual specificity for thyroglobulin and thyroperoxidase. Autoimmunity 11: 179, 1992.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Thyroid Laboratory, Division of Endocrinology, Hospital das Clínicas, University of Sao Paulo Medical School, Sao Paulo, Brazil

    M. Knobel, M. F. Barca, F. Pedrinola & G. Medeiros-Neto M.D.

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  1. M. Knobel
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  2. M. F. Barca
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  3. F. Pedrinola
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  4. G. Medeiros-Neto M.D.
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Knobel, M., Barca, M.F., Pedrinola, F. et al. Prevalence of anti-thyroid peroxidase antibodies in autoimmune and nonautoimmune thyroid disorders in a relatively low-iodine environment. J Endocrinol Invest 17, 837–842 (1994). https://doi.org/10.1007/BF03347788

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