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In Vitro Regeneration of a High Oil-yielding Variety of Safflower (Carthamus tinctorius var HUS-305)

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Abstract

A protocol for regular in vitro regeneration of Carthamus tinctorius var HUS-305 is described. The morphogenic response of seed explants and explants from seedlings of different ages were studied on Murashige and Skoog’s medium (MS) supplemented with different growth regulators. The protocol finally standardized involves culture of cotyledonary explants from 2 cm long, 2- to 6-day-old seedlings on MS supplemented with 1.0 mg l−1 BAP and 0.1 mg l−1 kinetin. Various other adjuvants viz., adenine sulphate, glutamine and casein hydrolysate were also tested. None of these promoted the caulogenic response significantly. The microshoots could be rooted on medium supplemented with different auxins. The highest rhizogenic response was on 1/2 MS supplemented with 0.2 mg l−1 NAA.

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Abbreviations

AdS:

Adenine Sulphate

kn:

Kinetin

BAP:

6-benzylaminopurine

NAA:

α-naphthalene acetic acid

2,4-D:

2,4-dichlorophenoxy acetic acid

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

CH:

Casein hydrolysate

MS:

Murashige and Skoog’s medium

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Authors and Affiliations

  1. Department of Botany, University of Delhi, Delhi, 110 007, India

    Neetika Walia, Amandeep Kaur & Shashi B. Babbar

Authors
  1. Neetika Walia
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  2. Amandeep Kaur
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  3. Shashi B. Babbar
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Corresponding author

Correspondence to Shashi B. Babbar.

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Walia, N., Kaur, A. & Babbar, S.B. In Vitro Regeneration of a High Oil-yielding Variety of Safflower (Carthamus tinctorius var HUS-305). J. Plant Biochem. Biotechnol. 14, 65–68 (2005). https://doi.org/10.1007/BF03263229

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  • DOI: https://doi.org/10.1007/BF03263229

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