Abstract
Multiple shoot induction in Litchi chinensis Sonn. (litchi) has been achieved by two methods: (1) direct germination of litchi seeds in 6-benzylaminopurine (20 mg l–1)-supplemented MS liquid medium and supported on a filter-paper bridge and (2) in planta treatment with 6-benzylaminopurine (100 μg on alternate days) of the axillary bud regions of plants germinated and maintained under sterile conditions. While the former method resulted in as many as 27.5±8.6 shoot buds from the cotyledonary node within 4 weeks, the latter yielded on average approximately 8 shoot buds from each treated node in 8 weeks. The cytokinin treatment in planta consisted of placing sterile filter paper moistened with sterile distilled water over the node and adding different concentrations of 6-benzylaminopurine. Both methods of multiple shoot induction were found to be effective for the five genotypes of litchi that were tested. The shoots elongated and rooted directly in vermiculite after a pulse treatment with IBA (25 mg/ml) for 15 min. Fungus growth which is a serious problem in litchi tissue culture, was controlled using a fungicide, Bavistin, and by eliminating organic nutrients from the growth medium.
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Received: 27 July 1998 / Revision received: 30 September 1998 / Accepted: 27 October 1998
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Das, D., Shiva Prakash, N. & Bhalla-Sarin, N. Multiple shoot induction and plant regeneration in litchi (Litchi chinensis Sonn.). Plant Cell Reports 18, 691–695 (1999). https://doi.org/10.1007/s002990050644
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DOI: https://doi.org/10.1007/s002990050644