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Differentiation of Isolates of Cotton Root Rot Pathogens Rhizoctonia solani and R. bataticola Using Pathogenicity and RAPD Markers

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Abstract

Twelve isolates of Rhizoctonia solani and fifteen isolates of R. bataticola causing root rot of cotton were studied for their pathogenicity and genetic diversity using RAPD markers. Pathogenicity of these isolates varied when tested on Gossypium arboreum variety RG-8. The similarity value of RAPD profiles in R. solani isolates ranged from 0.35 to 1.00 with an average of 0.67 among all the isolates and in R. bataticola it ranged from 0.27 to 1.00 with an average of 0.63 among all the isolates. Eighteen primers were used to fingerprint the individual isolates. The cluster analysis using unweighted primer group method with arithmetic average could distinguish R. solani and R. bataticola isolates into two separate fingerprint groups.

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Authors and Affiliations

  1. Central Institute for Cotton Research ( CICR ), Regional Station, Sirsa, 125 055, India

    D. Monga & S. S. Rathore

  2. Krishi Bhawan, New Delhi, 110 001, India

    C. D. Mayee

  3. National Research Centre on Plant Biotechnology, IARI, New Delhi, 110 012, India

    T. R. Sharma

Authors
  1. D. Monga
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  2. S. S. Rathore
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  3. C. D. Mayee
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  4. T. R. Sharma
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Correspondence to D. Monga.

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Monga, D., Rathore, S.S., Mayee, C.D. et al. Differentiation of Isolates of Cotton Root Rot Pathogens Rhizoctonia solani and R. bataticola Using Pathogenicity and RAPD Markers. J. Plant Biochem. Biotechnol. 13, 135–139 (2004). https://doi.org/10.1007/BF03263209

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  • DOI: https://doi.org/10.1007/BF03263209

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