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Regeneration of Plantlets via Callus Cultures in Celastrus paniculatus Willd-A Rare Endangered Medicinal Plant

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Abstract

Plantlet regeneration in Celastrus paniculatus Willd (Celastraceae), an endangered medicinal plant has been achieved from cotyledonary leaf-derived callus. The cotyledonary leaves from mature embryo callused on MS medium supplemented with NAA (5 × 10-6 M) and Kinetin (5 × 10-6 M) with a doubling time of 16.2 days. Well developed multiple shoots of 5.6 cm could be obtained via organogenesis within 28 days on MS + BA (2 × 10-5 M). Altered shoot morphology occurred with the change in the concentration of auxins and cytokinins. Rooting (85%) of isolated shoots was achieved on McCown medium (WPM) containing IBA (5 × 10-6 M). Rooted plantlets were transferred in small pots containing sand, garden soil and vermiculite mix. The procedure can serve as a reliable and reproducible protocol for propagation and ex situ conservation ofthis species.

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Authors and Affiliations

  1. Plant Tissue Culture Unit, Regional Research Laboratory (CSIR), Jammu Tawi, 180 001, India

    M. Sharada, Ashok Ahuja & M. K. Kaul

Authors
  1. M. Sharada
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  2. Ashok Ahuja
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  3. M. K. Kaul
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Corresponding author

Correspondence to Ashok Ahuja.

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RRL Contribution No. 2355

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Sharada, M., Ahuja, A. & Kaul, M.K. Regeneration of Plantlets via Callus Cultures in Celastrus paniculatus Willd-A Rare Endangered Medicinal Plant. J. Plant Biochem. Biotechnol. 12, 65–69 (2003). https://doi.org/10.1007/BF03263163

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  • DOI: https://doi.org/10.1007/BF03263163

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