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Micropropagation of Rollinia mucosa (JACQ.) baill

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Summary

A protocol for in vitro propagation of Rollinia mucosa, an important medicinal plant, was developed. The presence of 500 mg l−1 polyvinylpyrrolidone (PVP) during explant excision was important to avoid browning. Axillary buds, adventitious buds, and shoot cluster proliferation were achieved from epicotyl and hypocotyl explants from nursery-grown seedlings. The highest direct organogenesis percentage from hypocotyl explants was obtained upon culture of explants on Murashige and Skoog medium supplemented with 2.2 μM benzyladenine (BA) plus 2.32 μM kinetin. Epicotyl explants display highest regeneration frequency on a medium containing 8.8 μM BA and 0.54 μM naphthaleneacetic acid. Gibberellic acid was necessary for shoot elongation. Root induction was observed when shoots were pretreated with activated charcoal for 7 d in the dark before culture on Woody Plant Medium supplemented with 49.21 μM indolebutyric acid for 10 d. Root development was observed when 20 g l−1 sucrose was used. Rooted plantlets were acclimatized and grown in the greenhouse.

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Authors and Affiliations

  1. Laboratório de Biotecnologia de Plantas (LABPLAN), Instituto de Biologia Roberto Alcântara Gomes, Universidade do Estado do Rio de Janeiro (UERJ), Rua São Francisco Xavier, 524, PHLC, s/509, CEP-20550-013, Maracanã, RJ, Brazil

    Solange Faria Lua Figueiredo, Norma Albarello & Vera Regina Campos Viana

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  1. Solange Faria Lua Figueiredo
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  2. Norma Albarello
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  3. Vera Regina Campos Viana
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Correspondence to Solange Faria Lua Figueiredo.

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Figueiredo, S.F.L., Albarello, N. & Campos Viana, V.R. Micropropagation of Rollinia mucosa (JACQ.) baill. In Vitro Cell.Dev.Biol.-Plant 37, 471–475 (2001). https://doi.org/10.1007/s11627-001-0083-1

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  • DOI: https://doi.org/10.1007/s11627-001-0083-1

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