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Introduction and Expression of Cowpea Trypsin Inhibitor (CpTI) Gene in Transgenic Tobacco

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Abstract

A trypsin/chymotrypsin inhibitor (CpTI) was purified from mature cowpea seeds. A full length cDNA clone encoding the trypsin/chymotrypsin inhibitor protein was isolated from a cDNA library that was constructed using poly(A+) RNA from developing seeds. Tobacco protoplasts were transformed with a construct composed of CaMV 35S promoter, NPTII gene, CpTI gene, and NOS terminator, using polyethylene glycol (PEG)-mediated direct gene transfer. The CpTI gene has been integrated into the plant genome, and the expressed CpTI protein from transgenic plants was catalytically active.

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Author notes

  1. Durba Ghoshal

    Present address: Indian Institute of Technology, Kharagpur, India

Authors and Affiliations

  1. Plant Molecular and Cellular Genetics, Bose Institute, Calcutta, India

    Durba Ghoshal & S. K. Sen

  2. Laboratory of Plant Physiology, Biochemistry and Biotechnology, Department of Biology, and Department of Biochemistry and Molecular Biology, University of Minnesota Duluth, Duluth, MN, 55812, USA

    Durba Ghoshal & Arun Goyal

Authors
  1. Durba Ghoshal
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  2. S. K. Sen
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  3. Arun Goyal
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Corresponding author

Correspondence to Durba Ghoshal.

Additional information

The nucleotide sequence data has been submitted in the GenBank nucleotide sequence database under the accession number AF327406.

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Ghoshal, D., Sen, S.K. & Goyal, A. Introduction and Expression of Cowpea Trypsin Inhibitor (CpTI) Gene in Transgenic Tobacco. J. Plant Biochem. Biotechnol. 10, 19–24 (2001). https://doi.org/10.1007/BF03263101

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  • DOI: https://doi.org/10.1007/BF03263101

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