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Sequence Analysis of a Plastidial Omega-3 Desaturase Gene from Brassica juncea

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Abstract

A genomic clone (λg2) encoding a plastidial omega-3 desaturase has been isolated from a genomic library of Brassica juncea cv Pusa Bold and sequenced after subcloning. A continuous stretch of 3866 by of the clone sequenced showed homology with omega-3 desaturase from other plant sources but maximum homology was with that of a plastidial omega-3 fad 7 gene. This sequence designated as Bjgfad7 includes 1381 by 5’ untranslated region, 273 by 3’ untranslated region along with an open reading frame of 1290 by interrupted by 7 introns. The 5’ untranslated region contains putative cis elements responsible for seed specificity, light responsive and stress-inducible expression besides minimal promoter elements.

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Authors and Affiliations

  1. Division of Biochemistry, Indian Agricultural Research Institute, New Delhi, 110 012, India

    Renu Garg, I. M. Santha, M. L. Lodha & S. L. Mehta

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  1. Renu Garg
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  2. I. M. Santha
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  3. M. L. Lodha
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  4. S. L. Mehta
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Correspondence to I. M. Santha.

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Part of the Ph.D. Thesis submitted by the senior author to PG School, IARI.

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Garg, R., Santha, I.M., Lodha, M.L. et al. Sequence Analysis of a Plastidial Omega-3 Desaturase Gene from Brassica juncea . J. Plant Biochem. Biotechnol. 10, 13–17 (2001). https://doi.org/10.1007/BF03263100

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  • DOI: https://doi.org/10.1007/BF03263100

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