Abstract
Genetic variation in nine aromatic and four nonaromatic rice varieties (Oryza sativa L.) was investigated at the DNA level using the randomly amplified polymorphic DNA (RAPD) technique. Twenty six random primers were used to amplify DNA segments and 177 PCR products were obtained of which 98 were polymorphic. One primer did not show polymorphism. A dendrogram showing the genetic distances of 13 rice varieties was constructed based on RAPD data.
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Berner OK & Hoff BJ, Crop Sci, 26(1986) 876.
Brook D, Rice Situation and Outlook Report, (USDA-ERS-RS-56), US GPO, Washington DC. (1989) 11.
Vu LX & Nguyen HT, Theor Appl Genet, 87, (1994), 668.
Mackill DJ, Crop Sci, 35, (1995), 889.
Buso GSC, Rangel PH & Ferreira ME, Mol Ecol, 7, (1998), 107.
Qingsheng J, Boqin Q, Wencho Y & Rubi L, Acta Agriculturae Zhejiangensis. 7 (1995) 439.
Deliaporta SL, Wood J & Hichs JB, Plant Mol Biol Rep, 1, (1983), 19.
Sambrook J, Fritsch EF & Maniatis T, in Molecular Cloning: A Laboratory Manual 2nd Ed, Cold Spring Harbour Laboratory Press, Cold Spring, NY (1989).
Cao D & Card JH, Crop Sci. 37 (1997) 1630.
Vierling RA & Nguyen HT, Theor Appl Genet, 84, (1992), 835.
Virk PS, Newburry HJ, Jackson MT & Ford-Lloyd BV, Theor Appl Genet, 90, (1995), 1049.
Altschul, Stephen F, Gish W, Miller W, Myers EW & Lipman DJ, J Mol Biol, 215(1990), 403.
Ahn SN, Bollich CN & Tanksley SO, Theor Appl Genet, 84, (1992), 825.
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Baishya, S., Sachdev, A., Johari, R.P. et al. RAPD Analysis of Aromatic and Non-aromatic Rice (Oryza sativa L.). J. Plant Biochem. Biotechnol. 9, 23–26 (2000). https://doi.org/10.1007/BF03263078
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DOI: https://doi.org/10.1007/BF03263078