Abstract
Clonal propagation in vitro of raisin tree (Hovenia dulcis Thunb.) was achieved using axillary buds from mature trees and young plants. Explants cultured on Murashige-Skoog’s medium with 1/3 of the original salt concentration, supplemented with 0.5 mg l-1 BAP and 0.5 mg l-1 IAA, showed proliferation of new shoots in 4-5 weeks. Adventitious shoot proliferation was also stimulated in subsequent subcultures in the presence of BAP. The shoots rooted when transferred to 1/3 Murashlge and Skoog’s medium with 0.1 mg l-1 of IBA. Plantlets thus formed were successfully transplanted to the field after a short acclimatization period.
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Abbreviations
- BAP:
-
6-benzyl aminopurine
- IAA:
-
indole acetic acid
- IBA:
-
indole butyric acid
- NAA:
-
α-naphthalene acetic acid
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Echeverrigaray, S., Mossi, A.J. & Munari, F. Micropropagation of Raisin Tree (Hovenia dulcis Thunb.) Through Axillary Bud Culture. J. Plant Biochem. Biotechnol. 7, 99–102 (1998). https://doi.org/10.1007/BF03263043
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DOI: https://doi.org/10.1007/BF03263043