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Callus Induction from Explants of Crocus sativus

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Abstract

Saffron calli were induced from ovary explants on Murashige-Skoog (MS) medium supplemented with beyzyladenine (BA) and naphthalene acetic acid (NAA) as growth factors. MS medium with 5 mg l−1 BA and 10 mg l−1 NAA was selected for calli induction and undifferentiated calli growth, while MS medium with 1 mg l−1 BA and 1 mg l−1 NAA was the most appropriate for stigma differentiation. On this medium, stigma-like structures measuring 0.5–1.5 cm were obtained. Initially they were colourless, but yellow pigmentation, due to the presence of crocin, progressively increased with calli growth. Extracts of stigma-like structures were analysed by HPLC and the presence of saffron secondary metabolites was demonstrated. In addition, calli also showed yellow pigmentation.

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Authors and Affiliations

  1. Department of Biochemistry, Molecular Biology and Immunology, Faculty of Chemistry, University of Murcia, P.O. Box 4021, E-30100, Murcia, Spain

    M. R. Castellar & J. L. Iborra

Authors
  1. M. R. Castellar
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  2. J. L. Iborra
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Castellar, M.R., Iborra, J.L. Callus Induction from Explants of Crocus sativus . J. Plant Biochem. Biotechnol. 6, 97–100 (1997). https://doi.org/10.1007/BF03263018

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  • DOI: https://doi.org/10.1007/BF03263018

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