Abstract
Induction of high frequency of direct in vitro flowering (35.7%) from deembryonated cotyledons of groundnut cultivar TMV2 on a modified Blaydes’ medium with 0.5 mg/l 6-benzyladenine, provides a model system to study the gene expression. Gene homologue of ‘AGAMOUS’ in groundnut was detected as a single-copy gene and ‘AG’ RNA transcripts were observed as specific to floral tissue. RNA dot blot analysis indicated the stage specific expression of ‘AG’ gene in the presence of phytohormone, BA, which correlated with in vitro flower initiation process within 2–6 days of inoculation as suggested by histological studies. BA is associated with flower bud initiation through the activation of AGAMOUS gene.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Thomas B, The molecular biology of flowering (BR Jordam, Editor), CAB International, Oxon (1993) pp 1–20.
Bernier G, Havelange A, Houssa C, Petitjean A & Lejeune P, Plant Cell, 5 (1993) 1147.
Okamuro KK, der Boer BGW & Jofuku KID, Plant Cell, 5 (1993) 1183.
Yanofsky MF, Ma H, Bowman JL, Drews GN, Feldmann KA & Meyerowitz EM, Nature, 346 (1990) 35.
Meyerowitz EM, Proc Natl Acad Sci, USA, 91 (1994) 5735.
Shannon S & Meeks-Wagner DR, Plant Cell, 5 (1993) 639.
Meyerowitz EM, Personnel communication (1995).
Tran Than Van K, In Morphogenesis in plants (KA Roubeiakis-Angelakis, Editor), Plenum Press, New York (1993) pp 39–54.
Reddy GM, In New frontiers in breeding researches (B Napompeth, S Subhadrabandhu, Editors) Fac Agri Kasetsart University, Bangkok (1986) pp 97–102.
Narasimhulu SB & Reddy GM, Theor Appl Genet, 69 (1984) 87.
Narsimhulu SB & Reddy GM, In Genet structure and function in higher plants (GM Reddy, EH Cor Jr, Editors), Oxford and IBH, New Delhi (1986), pp 257–264.
Blaydes DF, Physiol Plant, 19 (1966) 599.
Pattee HE, Stalker HT & Giesbrecht FG, Bot Gaz, 152 (1991) 64.
Murray M & Thompson WF, Nucleic Acids Res, 8 (1980) 4321.
Schuler MA & Zielinski RE, Methods in plant molecular biology, Academic press, Inc., San Diego (1989) pp 8996.
Sambrook J, Fritsch EF & Maniatis T, Molecular cloning: A laboratory manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York (1989).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kumar, T.A., Reddy, G.M. Identification and Expression of AGAMOUS Gene Homologue During In Vitro Flowering from Cotyledons of Groundnut. J. Plant Biochem. Biotechnol. 6, 81–84 (1997). https://doi.org/10.1007/BF03263015
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF03263015