Abstract
Following microprojectile mediated delivery of a plasmid construct (pAHC-25) encoding bar (bialophos resistance) gene into five-day-old scutellar calli derived from mature embryos, the effectiveness of selection procedure for bar-gene expressing tissue was compared for two indica rice cultivars (IR-64 and Karnal Local). While IR-64 transformants could be selected through the generally used semi-solid selection medium, the same procedure was not effective in the basmati cultivar Karnal Local. In the latter case, while lower concentrations (2–4 mg 1−1) of the selective agent phosphinothricin (PPT) yielded only escapes, higher concentrations (6–8 mg l−1) inhibited proliferation of transformed as well as untransformed sectors. For Karnal Local, a liquid medium based selection system was successfully utilized for recovering transformed sectors and, eventually, regenerants. The study demonstrates the generation of transformants of two elite indica cultivars using the environment-independent system of mature embryos from seeds.
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Abbreviations
- 2,4-D:
-
2,4-Dichlorophenoxyacetic acid
- NAA:
-
α-naphthaleneacetic acid
- BAR:
-
6-benzylaminopurine
- K:
-
kinetin
- PPT:
-
Phosphinothricin
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Khanna, H., Raina, S.K., Srinivasulu et al. Biolistic Transformation of Elite Indica Rice (Oryza sativa L.) Cultivars Through Semi-Solid and Liquid Medium Selection Systems. J. Plant Biochem. Biotechnol. 6, 75–80 (1997). https://doi.org/10.1007/BF03263014
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DOI: https://doi.org/10.1007/BF03263014