Abstract
The microprojectile bombardment of immature embryos has proven to be effective in transforming many indica rice varieties. One of the drawbacks of using immature embryos is the requirement of a large number of high quality immature embryos, which itself is a tedious and laborious process. To circumvent these problems, we have developed a procedure, using indica variety TN1 as a model that generates highly homogenous populations of embryogenic subcultured calli by selectively propagating a small number of regeneration-proficient calli derived from seeds. Thousands of embryogenic calli were produced from 50 seeds within 10 weeks. Ten to 20 independent R0 transgenic lines were regenerated per 500 embryogenic calli bombarded. The convenience and reliability offered by this transformation system has made transformation of indica rice a routine procedure.
Similar content being viewed by others
Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxy acetic acid
- NAA:
-
naphthalene acetic acid
- BAP:
-
6-benzylaminopurine
- kb:
-
kilobase
- GUS:
-
β-glucuronidase
- X-gluc:
-
5-bromo-4-chloro-3-indolyl-β-D-glucuronide
- HPH:
-
hygromycin B phosphotransferase
References
Chan, M.-T., Chang, H.-H., Ho, S.-L., Tong, W.-F. and Yu, S.-M. (1993) Plant Mol. Biol. 22: 491–506.
Christensen, A. H., Sharrock, R. A. and Quail, P. H. (1992) Plant Mol. Biol. 18: 675–689.
Christou, P., Ford, T. L. and Kofron, M. (1991) Bio/Technology. 9: 957–962.
Chu C.C., Wang C.C., Sun, C.S., Hsu S.C., Yin, K.C., Chu, C.Y and Bi, F.Y. (1975) Sci. Sin. 18: 659–668.
Datta, S. K., Datta, K. and Potrykus, I. (1990) Plant Sci. 67: 83–88.
Gamborg O.L., Miller, R.A., and Ojima, K. (1968) J. Exp. Res. 50;151–158.
Ghosh Biswas, G. C., Burkhardt, P. K., Wünn, J., Klöti, A. and Potrykus, I. (1994) Plant Cell Reports 13(9): 528–532.
Glaszmann, J. C. (1987) Theoretical and Applied Genetics 74: 21–30.
Hiei, Y, Ohta, S., Komari, T. and Kumashiro, T. (1994) The Plant Journal 6: 271–282
Jefferson, R. A. (1987) Plant Molecular Biology Reporter 5: 387–405.
Li, L., Qu, R., Kochko de, A., Fauquet, C. and Beachy, R. N. (1993) Plant Cell Reports 12: 250–255.
Peng, J., Kononowicz, H. and Hodges, T. K. (1992) Theoretical and Applied Genetics. 83: 855–863.
Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989) in Molecular cloning, a laboratory manual. Cold Spring Harbor Laboratory Press. pp 9.31–9.55.
Shimamoto, K., Terada, R., Izawa, T. and Fujimoto, H. (1989) Nature 338: 274–276.
Tian, W., Rancé, I. and Sivamani, E. (1994) Chinese Journal of Genetics 21(3): 1–9.
Toriyama, K., Arimoto, Y., Uchimiya, H. and Hinita, K. (1988) Bio/Technology. 6: 1072–1074.
Vain, P., NcMullen, M.D., Finer, J.J. (1993) Plant Cell Reports 12: 84–88.
Xu, X. and Li, B. (1994) Plant Cell Reports 13: 237–242
Zhang, H. M., Yang, H., Rech, E. L., Golds, T. J., Davis, A. S., Mullighan, B. J., Cocking, E. C. and Davey, M. R. (1988) Plant Cell Reports 7: 379–384.
Zhang, W. and Wu, R. (1988) Theor. Appl. Genet. 76: 835–840.
Author information
Authors and Affiliations
Additional information
Communicated by I. K. Vasil
Rights and permissions
About this article
Cite this article
Sivamani, E., Shen, P., Opalka, N. et al. Selection of large quantities of embryogenic calli from indica rice seeds for production of fertile transgenic plants using the biolistic method. Plant Cell Reports 15, 322–327 (1996). https://doi.org/10.1007/BF00232364
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00232364