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Detection of Genetic Variation in Alternaria brassicae by RAPD Fingerprints

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Abstract

Genetic variation in fourteen isolates of Alternaria brassicae collected from different geographical regions of the world was determined by RAPD (random amplified polymorphic DNA) analysis. Twenty random primers were tried to amplify genomic DNA of A. brassicae. Based on the PCR (polymerase chain reaction) amplification of genomic DNA of A. brassicae with four oligonucleotide random primers, fingerprints were generated for each isolate and the amplifed products were compared. Using this technique, intra- and intercontinental genetic variation among isolates of A. brassicae could be distinguished.

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Authors and Affiliations

  1. Department of Agricultural, Food, and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada, T6G 2P5

    T. R. Sharma & J. P. Tewari

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  1. T. R. Sharma
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  2. J. P. Tewari
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Sharma, T.R., Tewari, J.P. Detection of Genetic Variation in Alternaria brassicae by RAPD Fingerprints. J. Plant Biochem. Biotechnol. 4, 105–107 (1995). https://doi.org/10.1007/BF03262963

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  • DOI: https://doi.org/10.1007/BF03262963

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