Summary
We have used a PCR-based technique, involving the random amplification of polymorphic DNA (RAPD), to assess genome variability between 21 isolates from F. solani f. sp. cucurbitae races 1 and 2. Based on RAPD marker patterns the isolates fell into two distinct groups corresponding to mating populations MPI and MPV. Four isolates that could not be assigned to one or other mating population by traditional means were distinguished by RAPD patterns. Seven polymorphic RAPD products were used to probe Southern blots of MPI and MPV genomic DNA. Six of the seven probes hybridized to single-copy sequences and five of the seven probes showed specificity for one or other mating population. We suggest that not only is the technique a rapid and reliable tool for isolate-typing of fungi but it also provides a rapid method for obtaining species- or racespecific hybridization probes.
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References
Burnett JH (1984) In: Moss MO, Smith JE (eds) The applied mycology of Fusarium. British Mycological Society, Cambridge, University Press, England, pp 39–70
Churchill ACL, Ciuffetti LM, Hansen DR, Van Etten HD, Van Alfen NK (1990) Curr Genet 17:25–31
Deconte R, Cuppens H, Marynen P, Cassiman JJ (1990) DNA and Cell Biol 9:461–469
Forster H, Oudermans P, Coffey MD (1990) Exp Mycol 14:18–31
Hulbert SH, Michelmore RW (1988) Molec Plant-Microbe Interact 1:17–24
Linz U (1990) Biotechniques 9:286–293
Love JM, Knight AM, McAleer MA, Todd JA (1990) Nucleic Acids Res 18:4123–4130
Martin FN, Kistler HC (1990) Exp Mycol 14:32–46
Matuo T, Snyder WC (1973) Phytopathology 63:562–565
McDonald BA, Martinez JP (1990) Curr Genet 17:133–138
Pennycook SR (1989) Plant diseases recorded in New Zealand, 3 vol. Plant Diseases Division, Department of Scientific and Industrial Research, Auckland
Saiki RK, Scharf S, Faloona F, Mullis KB, Horn GT, Erlich HA, Arnheim N (1985) Science 230:1350–1354
Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA (1988) Science 239:487–491
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Sarkar G, Kapelner S, Sommer SS (1990) Nucleic Acids Res 18:7465
Toussoun TA, Snyder WC (1961) Phytopathology 51:17–22
Welsh J, McClelland M (1990) Nucleic Acids Res 18:7213–7218
Williams JGK, Kubelick AR, Livak KJ, Rafalski JA, Tingey SV (1990) Nucleic Acids Res 18:6531–6535
Vogel HJ (1964) Amer Nat 98:435–446
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Communicated by J.D. Beggs
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Crowhurst, R.N., Hawthorne, B.T., Rikkerink, E.H.A. et al. Differentiation of Fusarium solani f. sp. cucurbitae races 1 and 2 by random amplification of polymorphic DNA. Curr Genet 20, 391–396 (1991). https://doi.org/10.1007/BF00317067
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DOI: https://doi.org/10.1007/BF00317067