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In Vitro Propagation of Gladiolus: Optimisation of Conditions for Shoot Multiplication

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Abstract

Effect of some physical and chemical factors on in vitro shoot multiplication in Gladioius was investigated. A modified MS medium with NH4NO3 reduced to half strength, KH2PO4 replaced with 300 mg/l of NaH2PO4 2 H2O and Kl omitted completely, was found to be better than the original MS. BAP was better than either kinetin or 2iP. Of the various physical factors studied, the propagule size, light and container volume influenced the rate of shoot multiplication. On the modified medium containing 0.5 mg/l BAP in dark, the propagules bearing 20 buds showed 8-fold multiplication.

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Author notes

  1. Prem Kumar Dantu

    Present address: Division of Biochemistry, Indian Agricultural Research Institute, New Delhi, 110 012, India

Authors and Affiliations

  1. Department of Botany, University of Delhi, Delhi, 110 007, India

    Prem Kumar Dantu & Sant S. Bhojwan

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  1. Prem Kumar Dantu
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  2. Sant S. Bhojwan
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Dantu, P.K., Bhojwan, S.S. In Vitro Propagation of Gladiolus: Optimisation of Conditions for Shoot Multiplication. J. Plant Biochem. Biotechnol. 1, 115–118 (1992). https://doi.org/10.1007/BF03262908

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  • DOI: https://doi.org/10.1007/BF03262908

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