Abstract
Although the genetic linkage map of Triticum tauschil, the D-genome progenitor of wheat its available, its use for linkage analysis of hexaploid wheat chromosome regions is hampered by the lack of polymorphism in wheat. Here we describe a strategy to identity probes that detect a high degree of polymorphism in wheat. The strategy involves the use of DNA probes that detect null alleles. About 16% of the Pstl genomic clones from Triticum tauschil detect null alleles in the species. The probes that detect null alleles reveal high degree of polymorphism among hexaploid wheat cultivars. The probes selected following this strategy are expected to detect null alleles throughout the tribe Triticeae, therefore, reveal high degree of polymorphism.
Similar content being viewed by others
References
Kihara H, Agric Hort (Tokyo), 19 (1944) 889.
McFadden ES & Sears ER, J Hered, 37 (1946) 81, 107.
Kam-Morgan LNW, Gill BS & Muthukrishnan S, Genome, 32 (1989) 724.
Chao S, Sharp PJ, Worland AJ, Warham EJ, Koebner R & Gale MD, Theor Appl Genet, 78 (1989) 495.
Gill KS, Lubbers EL, Gill BS, Raupp WJ & Cox TS, Genome, 34 (1991) 362.
Lubbers EL, Gill KS, Cox TS & Gill BS, Genome, 34 (1991) 354.
Chang C, Bowman JL, DeJohn AW, Lander ES & Meyerowitz EM, Proc Natl Acad Sci, USA, 85 (1988) 6856.
McCouch SR, Kochert G, Vu ZH, Wang ZV, Khush GS, Coffman WR & Tanksiey SO, Theor Appl Genet, 76 (1988) 815.
Gill KS & Gill BS, Genetics, 129 (1991) 257.
Helentjaris T, King G, Slocum M, Sledenstrang C & Wegman S, Plant Mol Biol, 5 (1985) 109.
Miller JC & Tanksley SO, Theor Appl Genet, 80 (1990) 385.
Landry BS, Kessell R, Leung H & Michelmore RW, Theor Appl Genet, 74 (1987) 646.
Havey MJ & Muehlbauer FJ, Theor Appl Genet, 77 (1989) 395.
Author information
Authors and Affiliations
Additional information
Contribution no. 92-549-J from the Kans Agric. Exp. Stn.
Rights and permissions
About this article
Cite this article
Gill, K.S., Gill, B.S. A Strategy to Identify Probes that Detect a High Degree of Polymorphism in Bread Wheat. J. Plant Biochem. Biotechnol. 1, 81–85 (1992). https://doi.org/10.1007/BF03262901
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF03262901