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Micro-patterned polydiacetylene vesicle chips for detecting protein-protein interactions

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Abstract

In conclusion, micro-patterned PDA vesicles were proved as a biosensor to detect a protein-protein interaction. The vesicles were conjugated with primary antibodies and immobilized on glass slides as an array form. The fluorescence level of the PDA vesicles was changed by proteinprotein interactions between primary and secondary antibodies. This experiment proves that the micro-patterned PDA vesicles are useful to detect protein level interactions and can serve as a novel substrate to develop a protein chip.

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Authors and Affiliations

  1. Department of Chemical and Biological Engineering, Korea University, 136-713, Seoul, Korea

    Kyung-Woo Kim, Hyun Choi, Gil Sun Lee, Dong June Ahn & Min-Kyu Oh

  2. Department of Chemical Engineering, Hanyang University, 133-791, Seoul, Korea

    Jong-Man Kim

Authors
  1. Kyung-Woo Kim
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  2. Hyun Choi
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  3. Gil Sun Lee
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  4. Dong June Ahn
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  5. Min-Kyu Oh
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  6. Jong-Man Kim
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Corresponding authors

Correspondence to Dong June Ahn or Min-Kyu Oh.

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Kim, KW., Choi, H., Lee, G.S. et al. Micro-patterned polydiacetylene vesicle chips for detecting protein-protein interactions. Macromol. Res. 14, 483–485 (2006). https://doi.org/10.1007/BF03219115

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  • DOI: https://doi.org/10.1007/BF03219115

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