Abstract
Recent increase of multi drug-resistant bacteria represents a crucial issue of public health. As novative approaches are required to face that problem, those emerging from nanotechnology are of great interest. In that context we propose the possibility to use gold nanoparticles combined with ionising radiation to destroy pathogenic bacteria. For that, we investigated the potential X-Rays enhanced reduction of bacterial cell viability, following nanoparticle exposure, on a bacterial model,Escherichia coli. Our first concern was to confirm the absence of toxicity of the colloidal solution used. Then, we developed an X-Ray irradiation system and showed that gold nanoparticles increased the efficiency of ionising radiation to induce bacteria cell death.
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Abbreviations
- CTAB:
-
Cetyl Trimethyl Ammonium Bromide
- DMEM:
-
Dulbecco’s Modified Essential Medium
- GNP:
-
Gold nanoparticle
- LB:
-
Luria Bertani
- MTT:
-
3-(4,5-dimethylthiazol-z-yl)-2,5-diphenytetrazotium bromide
- NIR:
-
Near-Infrared
- NP:
-
Nanoparticle
- PEG:
-
Polyethylene glycol
- TEM:
-
Transmission Electron Microscopy
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About the authors Angélique Simon-Deckers is a PhD student working at the Pierre Süe Laboratory, CEA-CNRS UMR 9956, Saclay, France. Her research focuses on biological effects of nanomaterials on in vitro cultured cells and on bacteria.
Emilie Brun is a PhD student working at the Laboratoire de Chimie Physique, Université Paris XI Orsay, CNRS UMR 8000, Orsay, France. Her research interest includes gold nanoparticle radiosensitizing effect.
Dr. Barbara Gouget was permanent researcher at the Pierre Süe Laboratory, CEA-CNRS UMR 9956, Saclay, France when these experiments were done. She is now working at the Scientific Department of the French Food Safety Agency, Maisons-Alfort, France.
Dr. Marie Carrière is permanent researcher at the Pierre Süe Laboratory, CEA-CNRS UMR 9956, Saclay, France. Her area of research includes human and ecotoxicity of environmental pollutants i.e. metals, radionuclides, and nanomaterials. Models employed arein vitro cultured cells, bacteria and plants cultivated in hydroponic solutions.
Dr. Cécile Sicard-Roselli is an Associate Professor in the Laboratoire de Chimie Physique, Université Paris XI Orsay, CNRS UMR 8000, Orsay, France. Her area of research includes radiobiology, analytical chemistry and nanosciences (gold nanoparticles).
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Simon-Deckers, A., Brun, E., Gouget, B. et al. Impact of gold nanoparticles combined to X-Ray irradiation on bacteria. Gold Bull 41, 187–194 (2008). https://doi.org/10.1007/BF03216596
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DOI: https://doi.org/10.1007/BF03216596