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The impairing effects of chaetochromin D on mitochondrial respiration and structure

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Abstract

Chaetochromin D, a toxic secondary metabolite ofChaetomlum graclle, was examined for impairing effects on mitochondrial respiration and structure (swelling-induction) using isolated rat liver mitochondria to gain Insight into the molecular mechanism for Its cytotoxicity. Chaetochromin D exerted similar mode of effects to those of chaetochromin A, cephalochromin, and ustilaginoldin A on mitochondrial reactions, causing uncoupling of oxidative phosphorylation, depression of state 3 respiration, and induction of drastic swelling In mitochondria.

Chaetochromin D induced the same style of swelling as that induced by chaetochromin A, being characterized by a very high rate and small amplitude of swelling. The swelling terminated In the middle and the amplitude was about half of the full swelling. Once the quick swelling ceased in the middle, subsequent swelling could not be elicited by the second addition of chaetochromin D at any of the concentrations tested.

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Authors and Affiliations

  1. Department of Biochemistry, Gifu University School of Medicine, Tsukasa-cho 40, 500, Gifu, Japan

    Satoru Mori & Yoshinori Nozawa

  2. Department of Food and Nutrition, Chukyo Women’s University, Yokone-cho, 474, Ohbu, Japan

    Klyoshi Kawai

  3. Department of Pharmacognosy, Meiji College of Pharmacy, Yato-cho, Tanashi, 188, Tokyo, Japan

    Kiyotaka Koyama & Shinsaku Natori

Authors
  1. Satoru Mori
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  2. Klyoshi Kawai
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  3. Yoshinori Nozawa
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  4. Kiyotaka Koyama
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  5. Shinsaku Natori
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Mori, S., Kawai, K., Nozawa, Y. et al. The impairing effects of chaetochromin D on mitochondrial respiration and structure. Mycotox Res 9, 85–93 (1993). https://doi.org/10.1007/BF03192239

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  • DOI: https://doi.org/10.1007/BF03192239

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