Summary
A sensitive and selective liquid chromatography-tandem mass spectrometric (LC-MS) method was developed and validated for the determination of Tramadol in human plasma and urine. The analyte was separated on a Diamonsil C18 column with ammonium acetate( 5mmol·L−1)-methanol(50:50, v:v) adjusted PH by caustic soda at a flow rate of 0.8ml min−1, and analyzed by mass spectrometry is in positive ion mode. The ion mass spectrum of m/z were 264.1 for Tramadol and 248.0 for Tinidazole (I.S.), respectively. The weighted (1/x2) calibration curve was linear over plasma concentration range 1.00–400.00ng/ml and urine concentration range 0.01–16.00 ng/ml, with a correlation coefficient (r) of 0.9995 and 0.9997, respectively. The lower limit of quantification in human plasma was 1.00ng/ml. The inter- and intra-day precisions (CV%) in both plasma and urine were lower than 10%, the mean method accuracies and recoveries from spiked plasma samples at three concentrations ranged from 98.2 to 100.1% and 61.6 to 62.9%, respectively. The developed method was successfully applied to determine Tramadol in human plasma and urine, and provided suitable profiles for clinical pharmacokinetic study of Tramadol.
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Liu, P., Liang, S., Wang, BJ. et al. Development and validation of a sensitive LC-MS method for the determination of tramadol in human plasma and urine. Eur. J. Drug Metabol. Pharmacokinet. 34, 185–192 (2009). https://doi.org/10.1007/BF03191172
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DOI: https://doi.org/10.1007/BF03191172