Abstract
Ethanolic extract of the defatted roots ofB. chitria was chromatographed when a compound-I (C21H25O5N) characterised as dihydropalmitinium hydroxide was isolated. Chronic administration of DPH for 20 days reduces the weights of ovary, uterus and vagina in normal cycling gerbils. Atresia of the large follicles and vacuolization of follicular cells was conspicuous. The luteal cells and endometrial glands were shrunken. Vaginal smear showed prolonged diestrous cycle reflecting suppression of estrogenic activity. Simultaneous administration of oestradiol dipropionate and DPH to spayed gerbils failed to maintain the growth of uterus and vagina. DPH treatment to intact/spayed/pregnant gerbils inhibited protein synthesis and decreased the sialic acid and glycogen contents of the genital tract. Serum protein was low whereas serum sialic acid, cholesterol, phospholipids, triglycerides and NEFA did not change. Antioestrogenic activity of the compound is discussed. Abortifacient action of DPH is of great significance in fertility regulation using plant products.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Arya M, Gupta S and Dixit V P 1979 Effect of cyproterone acetate on the reproductive system of the female rat;Acta Anat. 103 259–265
Bitman J, Cecil H C, Mench M L and Wren T R 1968 Kinetics ofin vivo glycogen synthesis in the oestrogen stimulated rat uterus;Endocrinology 76 63–66
]Chopra R N, Nayar S L and Chopra I C 1956Glossary of Indian medicinal plants (New Delhi: CSIR)
]Chopra R N, Chopra I C, Handa K L and Kapur L D 1958 Indigenous drugs of India (Calcutta: U N Dhur and Sons Pvt Ltd)
Clark J H, Anderson J N and Peck E J 1973 Estrogen receptor, antiestrogen complex: atypical binding by uterine nuclei and effects of uterine growth;Steroids 22 707–718
Coppola J A and Ball J L 1965 Uterine sialic acid in relation to ovarian steroids;Steroids 8 345–351
Drasher M L 1952 Morphological and chemical observations on the mouse uterus during the oestrous cycle and under hormonal treatment;J. Exp. Zool. 119 333–353
Dufour J, Cahill L P and Mauleon P 1979 Short and long-term effects of hypophysectomy and unilateral ovariectomy on ovarian follicular populations in sheep;J. Reprod. Fertil. 57 301–309
Galletti F and Gardi R 1973 Effects of ovarian hormones and synthetic progestins on vaginal sialic acid in rat;J. Endocrinol. 57 193–198
Gottfried S P and Rosenberg B 1973 Improved manual spectrophotometric procedure for determination of serum triglycerides;Clin. Chem. 19 1977–1978
Green C P 1978Voluntary sterilization world’s leading contraceptive method: Population reports special topic monographs NO 2, M-37
Gregoire A T, Ledger V D and Moran M J 1973 The glycogen content of the human female genital tract in cycling menopausal and women with endometrial and cervical carcinoma;Fertil. Steril. 24 181–201
Jones R C and Edgren R A 1973 The effects of various steroids on the vaginal histology in the rat;Fertil Steril. 24 284–291
Kamboj V P and Dhawan B N 1981 Current status of plants investigated for fertility regulation in India;Kor. J. Pharmacy. 12 111–118
]Kirtikar K R and Basu B D 1935Indian medicinal plants (Allahabad: Lalit Mohun Basu)
Lowry O H, Rosenbrough M T, Farr A L and Randall R J 1951 Protein measurement with folin phenol reagent;J. Biol. Chem. 193 265–275
Mohla S and Prasad M R N 1969 Oestrogen, antioestrogen interaction Effect of U 11100 A, MRL-41 (clomiphene) and U-11555 A on estrogen induced uterine glycogen and protein syntheses in rat during delayed implantation;Acta Endocrinol. 162 482–487
Montogomery R 1957 Determination of glycogen;Arch. Biochem. Biophys. 67 378–389
]Nadkarni A K and Nadkarni K M 1954Indian meteria medica (Bombay: Popular Book Depot)
Oliver M F and Boyd G S 1959 Effect of bilateral ovariectomy on coronary artery disease and serum levels;Lancet 2 690–694
]Oser B L 1965 In Hawk’sPhysiological chemistry 14th edn (New York: McGraw Hill) p. 246
Parrish H M, Carr C A and Hall D 1967 Time interval from the castration in premenopaused women to development of excessive coronary atherosclerosis;Am. J. Obstet. Gynaecol. 99 155–162
Punnonen R and Rauramo L 1974 Effect of oestrogen therapy on climacteric symptoms and tissue changes;Acta Obstet. Gynaecol. Scand. 53 267–269
Raj S G, Madhwa H G, Talbert L M and Dy R C 1981 Structural and functional expression of polycystic ovaries by Danazol;Fertil. Steril. 36 392–395
Soloni F G and Sardina L C 1973 Colorimetric micro-determination of free fatty acids;Clin. Chem. 19 419–424
Warren L 1959 The thiobarbituric acid assay of sialic acids;J. Biol Chem. 234 1971–1975
Zilversmit D B, Davis A K and Memphis Tenn 1950 Microdetermination of plasma phospholipids by trichloro-acetic acid precipitation;J. Lab. Clin. Med. 35 155–160
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Dixit, V.P., Gupta, R.S. Effects of dihydropalmitinium hydroxide isolated from the roots ofBerberis chitria on intact/spayed/oestradiol dipropiosate/pregnant female gerbils (Meriones hurrianae Jerdon). Proc Ani Sci 93, 629–637 (1984). https://doi.org/10.1007/BF03186313
Issue Date:
DOI: https://doi.org/10.1007/BF03186313