Abstract
Elicitor prepared from the cell walls ofPenicillium citrinum induces multiple responses ofTaxus chinensis cells, including nitric oxide (NO) generation, sequentially followed by the activation of PAL and synthesis of taxol. NO scavenger cPITO and nitric oxide synthase (NOS) inhibitor PBITU prevent the latter two reactions, all of which are triggered in the absence of elicitor by NO donor sodium nitroprusside (SNP). The elicitor-induced NO release ofTaxus chinensis suspension cells is strongly inhibited by PBITU. These results demonstrate a causal relationship between NO generation and the latter two reactions ofTaxus chinensis cells to the elicitor, and also indicate that NO, produced via NOS inTaxus chinensis cells treated with fungal elicitor, might act as an essential signaling molecule for triggering the activation of PAL and synthesis of taxol.
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Xu, M., Dong, J. & Zhu, M. Involvement of NO in fungal elicitor-induced activation of PAL and stimulation of taxol synthesis inTaxus chinensis suspension cells. Chin. Sci. Bull. 49, 1038–1043 (2004). https://doi.org/10.1007/BF03184034
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DOI: https://doi.org/10.1007/BF03184034