Abstract
A 500-bp cDNA fragment was amplified via RT-PCR from drought-induced total RNA of the drought-tolerantB. crassifolia Hemsl. using primers based on the sequence of published dehydrin conserved region. By using 5′ RACE, full-length coding region (1 148 bp) ofBDN1 gene was produced. It is a new member of the dehydrin gene family. Southern analysis indicated thatBDN1 is present in theB. crassifolia genome as a single-copy gene. Northern analysis revealed that its expression is inducible by drought and cold stresses as well as ABA application.
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Zhao, H., Liu, H., Yu, H. et al. Cloning and expression pattern of a dehydrin-likeBDN1 gene from drought-tolerantBoea crassifolia Hemsl.. Chin.Sci.Bull. 45, 2072–2077 (2000). https://doi.org/10.1007/BF03183529
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DOI: https://doi.org/10.1007/BF03183529