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Studies on isozyme variations in a few members ofDrosophila nasuta subgroup

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Proceedings: Animal Sciences

Abstract

Isozyme variation at six enzyme loci has been studied involving nine members ofDrosophila nasuta subgroup, by employing polyacrylamide gel electrophoretic technique. Alleles at three loci namely Acph, Aph and α-Est are found to be highly polymorphic; whereas at β-Est locus the alleles are less variable while at α-Gpdh and To loci, the alleles are found to be least variable. Null alleles are encountered in low frequencies at α-Est, β-Est and α-Gpdh enzyme loci. The allelic frequencies obtained at the six enzyme loci have been utilised as a yardstick to measure the extent of genetic relationship between the species studied. The genetic identity and genetic distance between these closely-related species have been estimated by adopting the Nei’s formula. These findings have been discussed with reference to earlier cytogenetic and hybridisation studies made on this subgroup.

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References

  • Ayala F J and Powell J R 1972 Allozymes as diagnostic characters of sibling species ofDrosophila;Proc. Natl. Acad. Sci. (USA) 69 1094–1096

    Article  CAS  Google Scholar 

  • Ayala F J and Tracey M L 1974 Genetic differentiation within and between species of theDrosophila willistoni group;Proc. Natl. Acad. Sci. (USA) 71 999–1003

    Article  CAS  Google Scholar 

  • Ayala F J, Powell J R and Dobzhansky Th 1971 Polymorphisms in continental and island populations ofDrosophila willistoni;Proc. Natl. Acad. Sci. (USA) 68 2480–2483

    Article  CAS  Google Scholar 

  • Ayala F J, Tracey M L, Hedgecock D and Richmond R C 1974a Genetic differentiation during the speciation process inDrosophila;Evolution 28 576–592

    Article  Google Scholar 

  • Ayala F J, Tracey M L, Barr L G and Ehrenfeld J G 1974b Genetic and reproductive diflerentiation of the subspecies,Drosophila equinoxialis caribbensis;Evolution 28 24–41

    Article  Google Scholar 

  • Ayala F J, Murao C A, Perez-Salas S, Richmond R C and Dobzhansky Th 1970 Enzyme variability in theDrosophila willistoni group. I. Genetic differentiation among sibling species;Proc. Natl. Acad. Sci. (USA) 67 225–232

    Article  CAS  Google Scholar 

  • Davis B J 1964 Disc electrophoresis. II. Method and application to human serum proteins;Ann. N.Y. Acad. Sci. 121 404–427

    Article  CAS  PubMed  Google Scholar 

  • Gillespic J H and Kojima K I 1968 The degree of polymorphisms in enzymes involved in energy production compared to that in non-specific enzymes in twoDrosophila ananassae populations;Proc. Natl. Acad. Sci. (USA) 61 582–585

    Article  Google Scholar 

  • Hansford R G and Sacktor B 1971 Oxidative metabolism of Insecta; inChemical zoology (eds) M Florkin and B T Scheer (New York, London: Academic Press) Vol. 6, pp. 213–247

    Google Scholar 

  • Hegde S N 1979Studies on the cytotaxonomy and geneties of a few members of the melanogaster species group of Drosophila; Doctoral dissertation, University of Mysore, Mysore, India

    Google Scholar 

  • Henning U and Yanofsky C 1963 An electrophoretic study of mutationally altered A proteins of the tryptophan synthetase ofEscherichia coli;J. Mol. Biol. 6 16–21

    Article  CAS  PubMed  Google Scholar 

  • Hubby J L and Throckmorton L H 1965 Protein differences inDrosophila. II. Comparative species genetics and evolutionary problems;Genetics 52 203–215

    CAS  PubMed  Google Scholar 

  • Hubby J L and Throckmorton L H 1968 Protein differences inDrosophila. IV. A study of sibling species;Am. Nat. 102 193–205

    Article  CAS  Google Scholar 

  • Hunter L R and Markert C L 1957 Histochemical demonstration of enzymes separated by zone electrophoresis in starch gels;Science 125 1294–1295

    Article  CAS  PubMed  Google Scholar 

  • Johnson F M 1971 Isozyme polymorphisms inDrosophila ananassae: Genetic diversity among island populations in the South Pacific;Genetics 68 77–95

    CAS  PubMed  Google Scholar 

  • Johnson F M, Kanapi C G, Richardson R H, Wheeler M R and Stone W S 1966 An operational classification ofDrosophila esterases for species comparisons;Univ. Texas Publ. 6615 517–532

    Google Scholar 

  • Kanapi C G and Wheeler M R 1970 Comparative isozyme patterns in three species of theDrosophila nasuta complex;Texas Rep. Biol. Med. 28 261–278

    CAS  Google Scholar 

  • King J L and Ohta T 1975 Polyallelic mutational equilibria;Genetics 79 681–691

    CAS  PubMed  Google Scholar 

  • Kojima K, Gillespie J and Tobari Y N 1970 A profile ofDrosophila species enzymes assayed by electrophoresis. I. Number of alleles, heterozygosities and linkage disequilibrium in glucose metabolising systems and some other enzymes;Biochem. Genet. 4 627–637

    Article  CAS  PubMed  Google Scholar 

  • Lakovaara S, Saura A, Lankinen P, Pohjola L and Lokki J 1976 The use of iosenzymes in tracing evolution and in classifying Drosophilidae;Zool. Scr. 5 173–179

    Article  Google Scholar 

  • Nair P S, Brncic D and Kojima K I 1971 Isozyme variations and evolutionary relationships in themesophragmatica group ofDrosophila;Univ. Texas Publ. 7103 17–28

    Google Scholar 

  • Nei M 1971 Interspecific gene differences and evolutionary time estimated from electrophoretic data on protein identity;Am. Nat. 105 385–398

    Article  CAS  Google Scholar 

  • Nei M 1972 Genetic distance between populations;Am. Nat. 106 283–292

    Article  Google Scholar 

  • Nirmala S S 1973Cytogenetic studies on the Drosophilids of Mysore State; Doctoral dissertation University of Mysore, Mysore, India

  • Nirmala S S and Krishnamurthy N B 1972Drosophila albomicans—a race ofDrosophila nasuta;Drosophila Inform. Serv. 49 60

    Google Scholar 

  • Nirmala S S and Krishnamurthy N B 1973–74 Cytogenetic studies onDrosophila neonasuta—A member of thenasuta subgroup.J. Mysore Univ. B26 162–167

    Google Scholar 

  • Ohta T and Kimura M 1975 Theoretical analysis of electrophoretically detectable polymorphisms: models of very slightly deleterious mutations;Am. Nat. 109 137–145

    Article  Google Scholar 

  • Prakash S 1977 Gene polymorphism in natural populations ofDrosophila persimilis;Genetics 85 513–520

    PubMed  Google Scholar 

  • Prakash S, Lewontin R C and Hubby J L 1969 A molecular approach to the study of genic heterozygosity in natural populations. IV. Patterns of genic variation in central marginal and isolated populations ofDrosophila pseudoobscura;Genetics 61 841–858

    CAS  PubMed  Google Scholar 

  • Rajasekarasetty M R, Ramesh S R and Krishnamurthy N B 1976 Studies on the polymorphism of α-Esterase activity in a few members ofnasuta subgroup (Genus:Drosophila);Entomon.1 17–21

    CAS  Google Scholar 

  • Rajasekarasetty M R, Ramesh S R and Krishnamurthy N B 1980 Interspecific chromosomal variation among a few members of thenasuta subgroup (Genus:Drosophila)—Entomon (in press)

  • Ranganath H A and Krishnamurthy N B 1976 Status ofDrosophila neonasuta in thenasuta subgroup;Egyptian J. Genet. Cytol. 5 141–145

    Google Scholar 

  • Ranganath H A, Rajasekarasetty M R and Krishnamurthy N B 1974 Evolutionary status of IndianDrosophila nasuta;Indian J. Heredity 6 19–25

    Google Scholar 

  • Rockwood E S, Kanapi C G, Wheeler M R and Stone W S 1971 Allozyme changes during the evolution of HawaiianDrosophila;Univ. Texas Publ. 7103 193–212

    Google Scholar 

  • Wright T R F 1961 The genetic control of an esterase inDrosophila melanogaster;Am. Zool. 1 476

    Google Scholar 

  • Wright T R F 1963 The genetics of an esterase inDrosophila melanogaster;Genetics 48 787–801

    CAS  PubMed  Google Scholar 

  • Yang S Y, Wheeler L L and Bock I R 1972 Isozyme variations and phylogenetic relationships in theDrosophila bipectinata species complex;Univ. Texas Publ. 7213 213–227

    Google Scholar 

  • Zouros E 1974 Genetic differentiation associated with the early stages of speciation in themulleri subgroup ofDrosophila;Evolution 27 601–621

    Article  Google Scholar 

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Ramesh, S.R., Rajasekarasetty, M.R. Studies on isozyme variations in a few members ofDrosophila nasuta subgroup. Proc. Indian Acad. Sci. (Anim. Sci.) 89, 197–213 (1980). https://doi.org/10.1007/BF03179161

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