Abstract
A cDNA and genomic clone (CuLEA5) encoding a group 5 late embryogenesis abundant protein (Lea5) was isolated from citrus fruit cDNA and genomic libraries. Sequence analysis indicated that the clone contains an open reading frame of 97 amino acids, and that the genomic structure is composed of two exons and one intron. A comparison of its amino sequence with other plant proteins showed that Lea5 proteins can be classified into two types - gymnosperm and angiosperm — based on a P-segment sequence designated by this study. Examination of its expression patterns indicated thatCuLEA5 has important roles during the development or ripening of seedless fruits and leaves inCitrus. The 5′-flanking region of the genomic DNA contains a number of putative hormonal- and stress-responsive elements. This is the first report that describes the expression ofLea5 during fruit ripening, as well as the sequence characteristics of its promoter region.
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Jeon, OS., Kim, CS., Lee, SP. et al. Fruit ripening-related expression of a gene encoding group 5 late embryogenesis abundant protein inCitrus . J. Plant Biol. 49, 403–408 (2006). https://doi.org/10.1007/BF03178819
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DOI: https://doi.org/10.1007/BF03178819