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Isolation and screening of marine associated bacteria from Tamil Nadu, Southeast coast of India for potential antibacterial activity

  • Ecological and Environmental Microbiology
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Abstract

The bacteria associated with living surfaces are rich sources of bioactive metabolites. In the present study, 182 heterotrophic epibacterial colonies, isolated from seaweeds (44%), ascidians (30.2%), barnacles (10.4%) and molluscan egg mass (15.4%), were subjected to high throughput screening by cross streaking method against six human pathogenic bacteria,Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Vibrio cholerae, Proteus mirabilis andKlebsiella pneumoniae. The 137 epibacterial isolates, which showed activity against at least one human bacterial pathogen in cross streaking method, were further cultured and the ethyl acetate extract of the culture broth (100 μg/disc) was assayed for antibacterial activity through disc diffusion method. The four epibacterial colonies, BR1, EM13, EM14 and PC4, isolated from the barnacleBalanus amphitrite, seaweedEnteromorpha compressa, and ascidianPolyclinum constellatum showed broad spectral antibacterial activity.

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Author notes

  1. Maniramakrishnan Santhana Ramasamy

    Present address: Laboratory for Cellular Organization and Signalling, National Centre for Biological Sciences (TIFR), Old Bellary Road, GKVK Campus, 560065, Bangalore, India

Authors and Affiliations

  1. School of chemical and biotechnology, Shanmugha Arts, Science, Technology & Research Academy, Sastra University, 613 402, Thanjavur, Tamil Nadu, India

    Subramanian Vijayalakshmi & Shanmugasudaram Murugesh

  2. Suganthi Devadason Marine Research Institute, 44 Beach Road, 628001, Tuticorin, Tamil Nadu, India

    Maniramakrishnan Santhana Ramasamy & Annappan Murugan

Authors
  1. Subramanian Vijayalakshmi
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  2. Maniramakrishnan Santhana Ramasamy
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  3. Shanmugasudaram Murugesh
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  4. Annappan Murugan
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Correspondence to Maniramakrishnan Santhana Ramasamy.

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Vijayalakshmi, S., Ramasamy, M.S., Murugesh, S. et al. Isolation and screening of marine associated bacteria from Tamil Nadu, Southeast coast of India for potential antibacterial activity. Ann. Microbiol. 58, 605–609 (2008). https://doi.org/10.1007/BF03175564

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  • DOI: https://doi.org/10.1007/BF03175564

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