Abstract
High-frequency electron paramagnetic resonance (HF-EPR) allows obtaining detailed information on the anisotropy of integer-spin systems, which are usually silent at conventional EPR frequencies (X- or Q-bands). However, the studies performed so far on integer-spin systems have mostly dealt with synthetic complexes. In this paper, we present first results obtained on a mononuclear high-spin ferrous iron center of a rubredoxin-type protein. This study shows that the broad-band quasi-optical technique of HF-EPR is a suitable approach towards the study of biologically relevant metal centers with large zero-field splittings.
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Barra, A.L., Hassan, A.K., Janoschka, A. et al. Broad-band quasi-optical HF-EPR spectroscopy: Application to the study of the ferrous iron center from a rubredoxin mutant. Appl. Magn. Reson. 30, 385–397 (2006). https://doi.org/10.1007/BF03166208
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DOI: https://doi.org/10.1007/BF03166208