Abstract
We measured the concentration and distribution of tumor associated antigens, TAG-72 and CEA, in stomach cancer byin vitro quantitative autoradiography (IV-QAR). Frozen sections of 33 specimens were incubated with varying concentrations of125I-labeled CEA-79.1 and B72.3 antibodies specific for carcinoembryonic antigen (CEA) and tumor-associated glycoprotein-72 (TAG-72), respectively. Computer analysis of specific antibody binding gave maximal binding values which were equal to the concentrations of the antigen or epitope. TAG-72 was detected in 25 specimens, at a concentration ranging from 8.4 to 562.9 pmol/g. CEA was detected in 32 of the 33 specimens and its concentration ranged from 8.8 to 525.3 pmol/g. The distribution of TAG-72 by IV-QAR coincided with that of the tumor cells in 41.4% of the pathologic lesions. The distribution of CEA coincided with the tumor cells in 80.5% of pathologic lesions, nearly twice the TAG-72. The concentration of TAG-72 was significantly higher in mucinous adenocarcinoma and mucin containing adenocarcinomas than other types of adenocarcinomas. There was no significant difference in the concentration of CEA among the pathologic types of stomach cancer. In summary, stomach cancer exhibited wide variations in TAG-72 and CEA expression. CEA expression was more frequent and homogeneous than TAG-72.
Similar content being viewed by others
References
Parkin DM, Stjernsward J, Muir CS. Estimates of the worldwide frequency of twelve major cancers.Bull WHO 62: 163–182, 1984.
Weed TE, Neessle W, Ochsner A. Carcinoma of the stomach: why are we failing to improve survival?Ann Surg 193: 407–413, 1981.
Diel JT, Hermann RE, Cooperman AM, Hoerr SO. Gastric carcinoma: a ten-year review.Ann Surg 198: 9–12, 1983.
Goldenberg DM, DeLand F, Kim E, Bennett S, Primus FJ, van Nagell JR, et al. Use of radiolabelled antibodies to carcinoembryonic antigen for the detection and localization of diverse cancers by external photoscanning.N Engl J Med 298: 1384–1388, 1978.
Goldenberg DM, Larson SM. Radioimmunodetection in cancer identification.J Nucl Med 33: 803–814, 1992.
Armitage NC, Perkins AC, Pimm MV, Farrands PA, Baldwin RW, Hardcastle JD, et al. The localization of an anti-tumor monoclonal antibody 791T/361 in gastrointestinal tumors.Br J Surg 71: 407–412, 1984.
Epenetos AA, Britton KE, Mather S, Shepherd J, Granowska M, Talyor-Papadimitriou J, et al. Targeting of iodine-123-labelled tumor-associated monoclonal antibodies to ovarian, breast and gastrointestinal tumors.Lancet 2: 999–1008, 1982.
Carrasquillo JA, Sugarbaker P, Colcher D, Reynolds JC, Esteban J, Bryant G, et al. Radioimmunoscintigraphy of colon carcinoma with iodine-131-labelled B72.3 monoclonal antibody.J Nucl Med 29: 1022–1030, 1988.
Wagner C, Muller-Wallraf R, Nisson S, Groener J, Breurer H. Localization and concentration of carcinoembryonic antigen (CEA) in gastrointestinal tumors: correlation with CEA levels in plasma.J Natl Cancer Inst 67: 539–547, 1981.
Estaban JM, Colcher D, Sugarbaker P, Schlom J. Quantitative and qualitative aspects of radiolocalization in colon cancer patients of intravenously administered MAb B72.3.Int J Cancer 39: 50–59, 1987.
Brummendorf T, Anderer FA, Staab HJ, Hornung A, Kieninger G. Carcinoembryonateantigen: diagnose der tumorprogression bei testinalen tumoren.Dtsch Med Wschr 110: 1963–1968, 1985.
Larson SM, Carrasquillo JA, Reynolds JC. Radioimmunodetection and radioimmunotherapy.Cancer Invest 2: 363–381, 1984.
Chung J-K, Graham K, Carrasquillo JA, Reynolds JC. Distribution and concentration of different epitopes on melanoma-associated antigens in human malignant melanoma.J Nucl Med 32: 1056, 1989.
Chung J-K, Lee DS, Lee MC, Koh C-S, Reynolds JC. Quantitative measurement of tumor-associated antigen by autoradiography.J Korean Cancer Associat 23: 740–748, 1991.
Colcher D, Horan Hand P, Nuti M, Schlom J. A spectrum of monoclonal antibodies reactive with human mammary tumor cells.Proc Nat Acad Sci (Wash) 78: 3199–3203, 1981.
Lee J-H, Chung H-K, Kimm S-W. Purification of carcinoembryonic antigen from culture supernatant of human colon cancer cell lines LS 174 T using monoclonal immuno-affinity chromatography.Korean J Biochem 20: 23–31, 1988.
Oh YS, Lee JH, Lee YH, Kim SR, Chung H-K, Tchai BS. Production and characterization of monoclonal antibodies to carcinoembryonic antigen.Korean J Biochem 20: 43–48, 1988.
Colcher D, Keenan AM, Larson SM, Schlom J. Prolonged binding of a radiolabeled monoclonal antibody (B72.3) used for thein situ radioimmunodetection of human colon carcinoma xenografts.Cancer Res 44: 5744–5751, 1984.
Chung J-K, Choi CW, Lee MC, Chung H-K, Kim NK, Choi KW, et al. Radioimmunoscintigraphy of advanced gastrointestinal carcinomas employing I-131 labeled CEA-79 monoclonal antibody.Ann Nucl Med 7: 65–70, 1993.
Mies G, Nievuhr I, Hossman KA. Simultaneous measurement of blood flow and glucose metabolism by autoradiographic technique.Stroke 12: 581–588, 1981.
Ohuchi N, Simpson JF, Colcher D, Schlom J. Complementation of anti-CEA and anti-TAG-72 monoclonal antibodies in reactivity to human gastric adenocarcinomas.Int J Cancer 40: 726–733, 1987.
Hockey MS, Stoker HJ, Thompson H, Woodhouse CS, MacDonald F, Fielding JWC, et al. Carcinoembryonic antigen expression and heterogeneity in primary and autologous metastatic gastric tumours demonstrated by a monoclonal antibody.Br J Cancer 49: 129–133, 1984.
Ejeckam GC, Huang SN, McCaughey WTE, Hold P. Immunohistopathologic study on carcinoembryonic antigen (CEA)-like material and immunoglobulin A in gastric malignancies.Cancer 44: 1606–1614, 1979.
Ohuchi N, Wunderlich D, Fujita J, Colcher D, Muraro R, Nose M, et al. Differential expression of carcinoembryonic antigen in early gastric adenocarcinomas versus benign gastric lesions defined by monoclonal antibodies reactive with restricted antigen epitopes.Cancer Res 47: 3565–3571, 1987.
Ohuchi N, Thor A, Nose M, Fujita J, Kyogoku M, Scholm J. Tumor-associated glycoprotein (TAG-72) detected in adenocarcinomas and benign lesions of the stomach.Int J Cancer 38: 643–650, 1986.
Gero EJ, Colcher D, Ferroni P, Melsheimer R, Giani S, Schlom J, et al. CA 72-4 radioimmunoassay for the detection of the TAG-72 carcinoma-associated antigen in serum of patients.J Clin Lab Anal 3: 360–369, 1989.
Del Vecchio S, Reynolds JC, Blasberg RG, Neumann RD, Carrasquillo JA, Hellstroem I, et al. Measurement of local Mr 97,000 and 250,000 protein antigen concentration in sections of human melanoma tumors usingin vitro quantitative autoradiography.Cancer Res 48: 5475–5481, 1988.
Nagura H, Asai T, Watanabe K. Immunocytochemical localization of secretory component (SC) and carcinoembryonic antigen (CEA) in human gastric mucosa.Acta Histochem Cytochem 14: 76, 1981.
Santeusanio G, Peronace L, Castagna G, De Muro G, Santi D, D’orazio A, et al. Immunohistochemical study of carcinoembryonic antigen (CEA) in gastric tumors: Correlation with preparative serum levels, histologic type and grade of anaplasia of the tumor.J Surg Oncol 37: 13–19, 1988.
Nielsen K, Teglbjaerg PS. Carcinoembryonic antigen (CEA) in gastric adenocarcinomas.Acta Path Microbiol Immunol Scand 90: 393–396, 1982.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Chung, JK., Lee, MC., Chung, HK. et al. Concentration and distribution of tumor associated antigens TAG-72 and CEA in stomach cancer. Ann Nucl Med 9, 7–13 (1995). https://doi.org/10.1007/BF03165002
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF03165002