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Radioimmunoscintigraphy of advanced gastrointestinal carcinomas employing I-131 labeled CEA-79 monoclonal antibody

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Abstract

CEA-79 is a murine IgG2a type monoclonal antibody (MoAb) generated using purified CEA from culture supernatants of a human colon cancer cell line, LS174T. The association constant and immunoreactivity of the I-131 labeled CEA-79 ranged from 2.0 to 3.2 ×109 l/mole, and from 54 to 74 %, respectively. The purpose of this study was to evaluate the feasibility of radioimmunoscintigraphy employing MoAb CEA-79 in patients with advanced gastrointestinal carcinomas. Two mgs of MoAb CEA-79 was labeled with 111 MBq (3 mCi) of I-131, and infused intravenously in 6 stomach cancer and 16 colon cancer patients. Out of 6 patients with stomach cancer, immunoscintigraphy was able to detect the tumors in 4 cases. However, immunoscintigraphy found out tumors in all patients with colon cancer. Moreover, 1 patient with stomach cancer and 2 patients with colon cancer showed increased uptake of MoAb in the tumor lesions despite normal serum levels of CEA. We could conclude that this antibody has a potential as a new imaging agent for the diagnosis of gastrointestinal carcinoma.

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Authors and Affiliations

  1. Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea

    June-Key Chung, Chang Woon Choi, Myung Chul Lee, Neo Kyeong Kim, Kyoo Wan Choi & Chang-Soon Koh

  2. Department of Biochemistry, Seoul National University College of Medicine, Seoul, Korea

    June-Key Chung, Hong-Keun Chung & Neo Kyeong Kim

  3. Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea

    Hong-Keun Chung

Authors
  1. June-Key Chung
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  2. Chang Woon Choi
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  3. Myung Chul Lee
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  4. Hong-Keun Chung
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  5. Neo Kyeong Kim
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  6. Kyoo Wan Choi
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  7. Chang-Soon Koh
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Additional information

This study was supported in part by a grant from the Cancer Research Center of Korea Scientific and Engineering Foundation. (grant number KOSEF SRC-56-CRC-11)

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Chung, JK., Choi, C.W., Lee, M.C. et al. Radioimmunoscintigraphy of advanced gastrointestinal carcinomas employing I-131 labeled CEA-79 monoclonal antibody. Ann Nucl Med 7, 65–70 (1993). https://doi.org/10.1007/BF03164570

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