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Evaluation of asialoglycoprotein receptor imaging agent as a marker of hepatic ischemia-reperfusion injury and recovery

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Abstract

Protection of hepatocytes from ischemia-reperfusion injury is a clinically important issue. The purpose of this study was to evaluate changes in acute liver damage and recovery after ischemiareperfusion in rats with asialoglycoprotein receptor (ASGP-R) ligand. Ischemia was induced by clamping the hepatoduodenal ligament for 90 min. At 1, 3,24, 48 hr, 1 and 2 wk after reperfusion, I-125-GSA was injected. Five min after injection, blood samples were obtained and the liver was removed. Several regions from each lobe were dissected, weighed and counted. Mean uptakes (% dose/g) in the liver and blood samples were calculated. Histologic sections stained with hematoxylin-eosin (H-E) stain showed ischemic damage at 1 and 3 hr, and focal hepatocyte necrosis at 24 hr. Predominant massive necrosis was not seen. The mitotic index with H-E stain and proliferating cell nuclear antigen (PCNA) labeling index were highest at 1 wk, indicating liver regeneration. At 1 and 3 hr, liver uptake was significantly decreased, and blood uptake was significantly increased, indicating decreased tissue blood flow and ischemic damage. Liver uptake showed significant increases at 48 hr and 1 wk, and was the highest at 1 wk, indicating liver regeneration during the convalescence stage. ASGP-R binding may provide valuable information on ischemia-reperfusion injury and recovery.

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Authors and Affiliations

  1. Department of Radiology, Fujita Health University, 1-98, Dengakugakubo, Kutsukake, 470-1192, Toyoake, Aichi, Japan

    Hiroshi Toyama, Kazuo Suzuki, Aiko Naito, Kaoru Kikukawa, Kazutaka Ejiri, Kohei Senda, Akira Takeuchi & Sukehiko Koga

  2. Department of Pathology, Fujita Health University, Toyoake

    Makoto Kuroda

  3. First Department of Surgery, Fujita Health University, Toyoake

    Yoshiyuki Komori & Akitake Hasumi

  4. Mie University, Tsu

    Kaname Matsumura

  5. Hokushin General Hospital, Nakano

    Toshiteru Fujiwara & Kiyonobu Ito

Authors
  1. Hiroshi Toyama
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  2. Kazuo Suzuki
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  3. Aiko Naito
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  4. Makoto Kuroda
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  5. Kaoru Kikukawa
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  6. Yoshiyuki Komori
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  7. Akitake Hasumi
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  8. Kaname Matsumura
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  9. Toshiteru Fujiwara
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  10. Kiyonobu Ito
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  11. Kazutaka Ejiri
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  12. Kohei Senda
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  13. Akira Takeuchi
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  14. Sukehiko Koga
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Corresponding author

Correspondence to Hiroshi Toyama.

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Toyama, H., Suzuki, K., Naito, A. et al. Evaluation of asialoglycoprotein receptor imaging agent as a marker of hepatic ischemia-reperfusion injury and recovery. Ann Nucl Med 13, 155–160 (1999). https://doi.org/10.1007/BF03164855

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  • DOI: https://doi.org/10.1007/BF03164855

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