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Concepts in high-frequency EPR — Applications to bio-inorganic systems

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Abstract

In this contribution we describe a high-frequency high-field EPR facility which has been developed at the University of Nijmegen. We present the design of a heterodyne quasi-optical bridge based on a millimeter-wave vector network analyzer as source and detection system. The mm-waves are transported in free-space through Gaussian beam optic elements and through a corrugated guide inside the resonator insert. The Fabri-Pérot (TEM00n) resonator is coupled through a metallic mesh and because of its bimodal property it can be operated using orthogonal detection leading to substantial improvement in sensitivity. In the first stage of the project, a multifrequency CW-facility is realized covering the 100–500 GHz range. In our initial explorative experiments we demonstrate the advantages of HF-EPR of high-spin systems: Due to the large microwave quantum, transitions which would be undetectable at X-band due to the large zerofield splitting can now be observed in good sensitivity. As a model for biological high-spin systems a sample of metmyoglobin was measured at D-band (130 GHz). Theg = 5.9 perpendicular line from theS = 5/2 ferric heme was detected and its line-width was compared to data previously obtained at Q-, X-, S- and L-band. As a model for biological integer spin systems theS = 1 signal of Ni(II) in nickel Tutton salt (Ni(NH4)2(SO4)2) was studied at 35 and 130 GHz.

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Authors and Affiliations

  1. Department of Molecular Spectroscopy, University of Nijmegen, Nijmegen, The Netherlands

    E. J. Reijerse, P. J. van Dam, A. A. K. Klaassen & W. R. Hagen

  2. High Field Magnet Laboratory, University of Nijmegen, Nijmegen, The Netherlands

    P. J. M. van Bentum

  3. School of Physics and Astronomy, University of St. Andrews, North Haugh, St. Andrews, Scotland, UK

    G. M. Smith

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  1. E. J. Reijerse
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  2. P. J. van Dam
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  3. A. A. K. Klaassen
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  4. W. R. Hagen
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  5. P. J. M. van Bentum
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  6. G. M. Smith
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Reijerse, E.J., van Dam, P.J., Klaassen, A.A.K. et al. Concepts in high-frequency EPR — Applications to bio-inorganic systems. Appl. Magn. Reson. 14, 153–167 (1998). https://doi.org/10.1007/BF03161887

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