Abstract
Effect of gibberellic acid was studied on nitrate utilization inSesamum seedlings grown under continuous light in standard mineral medium. Gibberellic acid treatment markedly inhibited nitrate uptake and its reduction. Sucrose 2% (w/v), when added in the incubation medium, significantly reversed this inhibition. Further, sugars and chlorophyll contents were also low and Hill-reaction activity recorded inhibition in GA3 treated seedlings. The poor uptake and reduction of nitrate is a result of low level of tissue sugars in gibberellic acid treated seedlings.
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References
Arnon D I 1949 Copper enzyme in isolated chloroplasts Polyphenol oxidase inBeta vulgaris;Plant Physiol. 24 1–15
Aslam M and Huffaker R 1984 Dependency of nitrate reduction on soluble carbohydrate in primary leaves under anaerobic condition;Plant Physiol. 75 623–628
Aslam M and Oaks A 1975 Effect of glucose on the induction of nitrate reductase in corn roots;Plant Physiol. 56 634–649
Aslam M, Oaks A and Huffaker R C 1976 Effect of light and glucose on the induction of nitrate reductase and on the distribution of nitrate in etiolated barley leaves;Plant Physiol. 58 588–591
Aslam M, Oaks A and Huffaker R C 1979 Effect of light and glucose on induction of nitrate reductase and on the distribution of nitrate in etiolated barley leaves;Plant Physiol. 58 588–591
Aslam M, Rosichan J L and Huffaker R 1987 Comparative induction of nitrate reductase by nitrate and nitrite;Plant Physiol. 83 579–584
Beevers L and Hageman R H 1969 Nitrate reduction in higher plants;Annu. Rev. Plant Physiol. 20 495–522
Breteler H, Hanisch ten Cate Ch H and Nissen P 1979 Time course of nitrate uptake and nitrate reductase activity in nitrogen-depleted dwarf bean;Physiol. Plant. 47 49–55
Breteler H and Hanisch ten Cate Ch H 1980 Fate of nitrate during initial nitrate utilization by nitrogen depleted dwarf bean;Plant Physiol. 48 292–296
Breteler H and Nissen P 1982 Effect of exogenous and endogenous nitrate concentration in nitrate utilization by dwarf bean;Plant Physiol. 70 754–759
Doddema H, Hoffstra J J and Feenstra W J 1978 Uptake of nitrate by mutant ofarabidopsis thaliana, disturbed in uptake and reduction of nitrate and chlorate;Physiol. Plant. 43 343–350
Fishman M J, Skougstad M W and Scarbra G F Jr 1964 Diazotization method for nitrate to nitrite;J. Am. Water Work Assoc. 56 633–638
Guerrero M G, Vega J M and Losada M 1981 The assimilatory nitrate reducing system and its regulation;Annu. Rev. Plant Physiol. 32 169–204
Jackson W A, Johnson R E and Volk R J 1974 Nitrate uptake by nitrogen-depleted wheat seedlings;Physiol. Plant. 32 37–42
Jackson W A 1978 Nitrate acquisition and assimilation by higher plants; inNitrogen in the environment (eds) D R Nielsen and J G MacDonald (New York: Academic Press) vol 2, pp 45–88
Jones P W, Johnson C B and Whittington W J 1981 Nitrate uptake and the induction of nitrate reductase activity in wheat (Triticum aestivum) seedlings;J. Exp. Bot. 32 9–18
Knypl J S 1979 Hormonal control of nitrate assimilation: Do phytohormones and phytochromes control the activity of nitrate reductase?; inNitrogen assimilation in plants (eds) E J Hewitt and C V Cutting (London: Academic Press) pp 541–556
Knypl J S and Janas K M 1979 Light and molybdenum requirement for substrate induction of nitrate reductase (EC 1·6·6·1) in cotyledons of LettuceLactuca sativa;Biol. Plant 21 214–219
Lüttge U and Higinbotham N 1979 Phytochrome and phytohormones affecting membrane transport mechanism; inTransport in plants (New York, Heidelberg, Berlin: Springer Verlag) pp 238–256
Miller G L 1959 Use of dinitrosalycylic acid reagent for determination of reducing sugar;Anal. Chem. 31 426–428
Naik M S, Abrol Y P, Nair T V R and Rao C S R 1982 Nitrate assimilation. Its regulation and relationship to reduced nitrogen in higher plants;Phytochemistry 21 495–504
Neyra C A, Franco A A and Pereira J C 1977 Seasonal pattern of nitrate reductase and nitrogenase activities inPhaseolus vulgaris (L.);Plant Physiol. 59 86–127
Rao K P and Rains D W 1976 Nitrate absorption by barley. I. Kinetics and energetics;Plant Physiol. 57 55–58
Ryle G J A, Powel C A and Cordon A J 1978 Effect of source of nitrogen on the growth of Fiskeby Soya bean: the carbon economy of whole plants;Ann. Bot. 42 637–648
Shaner D L and Boyer J S 1976 Nitrate reductase activity in maize (Zea mays L.) leaves. I. Regulation by nitrate flux;Plant Physiol. 58 499–504
Smith D 1969 Removing and analysing total nonstructural carbohydrates from plant tissue;Univ. Wisc. Res. Rep. No 41
Stewart G R 1972 End product repression of nitrate reductase inLemna minor (L.);Symp. Biol. Hung. 13 127–135
Vishniac W 1957 Methods for study of the Hill reaction;Methods Enzymol. 4 342–355
Zink M W 1982 Regulation of nitrate reductase by various nitrogen sources in culturedIpomoea sps.;Can. J. Bot. 60 386–396
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Bashist, D.P. Effect of gibberellic acid on nitrate utilization inSesamum indicum seedlings. Proc. Indian Acad. Sci. 98, 419–424 (1988). https://doi.org/10.1007/BF03053444
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DOI: https://doi.org/10.1007/BF03053444