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Light and molybdenum requirements for substrate induction of nitrate reductase in cotyledons of lettuce,Lactuca sativa L.

  • Original Papers
  • Published:
Biologia Plantarum

Abstract

Light was required for induction of nitrate reductase (NR, E.C. 1.6.6.1) in intact cotyledons of 2-day old seedlings ofLactuca sativa L. Molybdate strongly enhanced efficiency of induction. Benzyladenine (BA), gibberellin, and succinic acid-2,2-dimethylhydrazide reduced the enzyme activity. BA thrice enhanced incorporation of labelled leucine to the protein fraction. (2-chloroethyl)trimethylammonium chloride did not affect NR activity and markedly inhibited greening and protein synthesis. KNO3 stimulated protein synthesis as well as growth of the cotyledons.

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Abbreviations

Alar or B-9:

succinic aoid-2,2-dimethylhydrazide

BA:

N6-benzyladenine

CCC:

(2-ohloroethyl)trimethylammonium chloride

CAP:

chloramphenicol

CHI:

cycloheximide

FU:

5-fluorouraoil

GA3 :

gibberellic acid

NR:

nitrate reduotase

NRA:

NR activity

References

  • Beevers, L., Hageman, R. H.: The role of light in nitrate metabolism in higher plants. - Photophysiology7: 85–113, 1972.

    CAS  PubMed  Google Scholar 

  • Beevebs, L., Schrader, D., Flesher, D., Hageman, R. H.: The role of light and nitrate in the induction of nitrate reductase in radish cotyledons and maize seedlings. - Plant Physiol.40: 691–698, 1965.

    Article  Google Scholar 

  • Evans, W. R., Smillie, R. M.: Inhibition of light-dependent development of chloroplasts by 5-fluorouracil. - J. exp. Bot.22: 371–381, 1971.

    Article  CAS  Google Scholar 

  • Hewitt, E. J., Hucklesby, D. P., Manu, A. F., Notton, B. A., Rucklidge, G. J.: Regulation of nitrate assimilation in plants. - Nitrogen Assimilation of Plants, Proc. VI Long Ashton Symp., Sept. 19–22, 1977. In press, 1979.

  • Ingle, J.: Nucleic acid and protein synthesis associated with the induction of nitrate reductase in radish cotyledons. -Biochem. J.108: 715–724, 1968.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Johnson, C. B.: Rapid activation by phytochrome of nitrate reductase in the cotyledons ofSinapis alba. - Planta128: 127–131, 1976.

    Article  CAS  PubMed  Google Scholar 

  • Jones, R. W., Sheard, R. W.: Phytochrome, nitrate movement, and induction of nitrate reductase in etiolated pea terminal buds. - Plant Physiol.55: 954–959, 1975.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Klepper, L., Flesher, D., Hageman, R. H.: Generation of reduced nicotinamide adenine dinucleotide for nitrate reduction in green leaves. - Plant Physiol.48: 580–590, 1971.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Knypl, J. S.: Synergistic induction of nitrate reductase activity by nitrate and benzylaminopurine in detached cucumber cotyledons. - Z. Pflanzenphysiol.70: 1–11, 1973.

    Article  CAS  Google Scholar 

  • Knypl, J. S.: Hormonal control of nitrate assimilation: Do phytohormones and phytochrome control the activity of nitrate reductase? - Nitrogen Assimilation of Plants, Proc. VI Long Ashton Symp., Sept. 19–22, 1977. In press, 1979.

  • Knypl, J. S., Chylinska, K. M.: The inhibitory effect of (2-chloroethyl)trimethylammonium chloride on chlorophyll and protein synthesis in lettuce cotyledons, and its reversal by potassium. - J. exp. Bot.75: 525–529, 1972.

    Article  Google Scholar 

  • Nasrulhag-Boyce, A., Jones, O. T. G.: The light-induced development of nitrate reductase in etiolated barley shoots: An inhibitory effect of laevulinic acid. - Planta137: 77–84, 1977.

    Article  Google Scholar 

  • Notton, B. A., Hewitt, E. J.: Structure and properties of higher plant nitrate reductase especiallySpinacia oleracea L. - Nitrogen Assimilation of Plants, Proc. VI Long Ashton Symp., Sept. 19–22, 1977. In press, 1979.

  • Penot, M., Hourmant, A.: Effect of some phytohormones on cell permeability of potato storage tissue. - In:Sumiki, Y. (ed.): Plant Growth Substances 1973. Pp. 737–751. Hirokawa Publ. Co., Inc., Tokyo 1974.

    Google Scholar 

  • Ritenour, G. L., Joy, K. W., Bunning, J., Hageman, R. H.: Intracellular localization of nitrate reductase, nitrite reductase, and glutamic acid reductase in green tissue. - Plant Physiol.42: 233–237, 1967.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Sahulka, J.: The effect of exogenous IAA and kinetin on nitrate reductase, nitrite reductase, and glutamate dehydrogenase activities in excised pea roots. - Biol. Plant. B: 330–336, 1972.

    Article  Google Scholar 

  • Stulen, I., Koch-Bosma, T., Koster, A.: An endogenous inhibitor of nitrate reductase in radish cotyledons. - Acta Bot. Nèerl.20: 389–396, 1971.

    Article  CAS  Google Scholar 

  • Stulen, I., Lanting, L.: Nitrate reduotase and nitrite reductase activity in dark-grown radish seedlings: Relation to the supply of NADPH. - Physiol. Plant.37: 139–142, 1976.

    Article  CAS  Google Scholar 

  • Travis, R. L., Key, J. L.: Correlation between polyribosome level and the ability to induce nitrate reductase in dark-grown corn seedlings. - Plant Physiol.48: 617–620, 1971.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Vazquez, D.: Inhibitors of protein synthesis. - FEBS Letters40 Suppl.: S63-S84, 1974.

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Laboratory of Plant Growth Substances, University of Lodz, Ul. Banacha12/16, 90-237, Lodz, Poland

    J. S. Knypl & Krystyna M. Janas

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  1. J. S. Knypl
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  2. Krystyna M. Janas
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Knypl, J.S., Janas, K.M. Light and molybdenum requirements for substrate induction of nitrate reductase in cotyledons of lettuce,Lactuca sativa L.. Biol Plant 21, 214–219 (1979). https://doi.org/10.1007/BF02884572

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